Cao Shu-Guang, Wu Wan-Chun, Han Zhen, Wang Meng-Ya
Department of Gastroenterology, Yijishan Hospital, Wuhu 241001, Anhui Province, China.
World J Gastroenterol. 2005 Feb 7;11(5):737-40. doi: 10.3748/wjg.v11.i5.737.
To investigate the effects of psychological stress on small intestinal motility and expression of cholecystokinin (CCK) and vasoactive intestinal polypeptide (VIP) in plasma and small intestine, and to explore the relationship between small intestinal motor disorders and gastrointestinal hormones under psychological stress.
Thirty-six mice were randomly divided into psychological stress group and control group. A mouse model with psychological stress was established by housing the mice with a hungry cat in separate layers of a two-layer cage. A semi-solid colored marker (carbon-ink) was used for monitoring small intestinal transit. CCK and VIP levels in plasma and small intestine in mice were measured by radioimmunoassay (RIA).
Small intestinal transit was inhibited (52.18+/-19.15% vs 70.19+/-17.79%, P<0.01) in mice after psychological stress, compared to the controls. Small intestinal CCK levels in psychological stress mice were significantly lower than those in the control group (0.75+/-0.53 microg/g vs 1.98+/-1.17 microg/g, P<0.01), whereas plasma CCK concentrations were not different between the groups. VIP levels in small intestine were significantly higher in psychological stress mice than those in the control group (8.45+/-1.09 microg/g vs 7.03+/-2.36 microg/g, P<0.01), while there was no significant difference in plasma VIP levels between the two groups.
Psychological stress inhibits the small intestinal transit, probably by down-regulating CCK and up-regulating VIP expression in small intestine.
探讨心理应激对小肠动力及血浆和小肠中胆囊收缩素（CCK）和血管活性肠肽（VIP）表达的影响，以及心理应激状态下小肠运动障碍与胃肠激素之间的关系。
将36只小鼠随机分为心理应激组和对照组。通过将小鼠与饥饿的猫放置在双层笼子的不同层来建立心理应激小鼠模型。使用半固体彩色标记物（碳墨）监测小肠转运。采用放射免疫分析法（RIA）测定小鼠血浆和小肠中CCK和VIP水平。
与对照组相比，心理应激小鼠的小肠转运受到抑制（52.18±19.15%对70.19±17.79%，P<0.01）。心理应激小鼠小肠CCK水平显著低于对照组（0.75±0.53μg/g对1.98±1.17μg/g，P<0.01），而两组间血浆CCK浓度无差异。心理应激小鼠小肠中VIP水平显著高于对照组（8.45±1.09μg/g对7.03±2.36μg/g，P<0.01），而两组间血浆VIP水平无显著差异。
心理应激可能通过下调小肠中CCK表达和上调VIP表达来抑制小肠转运。
