Rodnina Marina V, Gromadski Kirill B, Kothe Ute, Wieden Hans-Joachim
Institute of Physical Biochemistry, University of Witten/Herdecke, 58448 Witten, Germany.
FEBS Lett. 2005 Feb 7;579(4):938-42. doi: 10.1016/j.febslet.2004.11.048.
Aminoacyl-tRNA (aa-tRNA) is delivered to the ribosome in a ternary complex with elongation factor Tu (EF-Tu) and GTP. The stepwise movement of aa-tRNA from EF-Tu into the ribosomal A site entails a number of intermediates. The ribosome recognizes aa-tRNA through shape discrimination of the codon-anticodon duplex and regulates the rates of GTP hydrolysis by EF-Tu and aa-tRNA accommodation in the A site by an induced fit mechanism. Recent results of kinetic measurements, ribosome crystallography, single molecule FRET measurements, and cryo-electron microscopy suggest the mechanism of tRNA recognition and selection.
氨酰-tRNA（aa-tRNA）以与延伸因子Tu（EF-Tu）和GTP形成的三元复合物形式被递送至核糖体。aa-tRNA从EF-Tu逐步移动到核糖体A位点涉及多个中间体。核糖体通过对密码子-反密码子双链体的形状识别来识别aa-tRNA，并通过诱导契合机制调节EF-Tu的GTP水解速率以及aa-tRNA在A位点的容纳速率。动力学测量、核糖体晶体学、单分子荧光共振能量转移测量和冷冻电子显微镜的最新结果揭示了tRNA识别和选择的机制。
