Pauli Daniela, Seyfarth Michael, Dibbelt Leif
Central Laboratory, Department of Clinical Chemistry, UK S-H, Campus Luebeck, Luebeck, Germany.
Clin Lab. 2005;51(1-2):31-41.
Applying basic potentiometric and photometric assays, we evaluated the fully automated random access chemistry analyzer Architect c8000, a new member of the Abbott Architect system family, with respect to both its analytical and operational performance and compared it to an established high-throughput chemistry platform, the Abbott Aeroset. Our results demonstrate that intra- and inter-assay imprecision, inaccuracy, lower limit of detection and linear range of the c8000 generally meet actual requirements of laboratory diagnosis; there were only rare exceptions, e.g. assays for plasma lipase or urine uric acid which apparently need to be improved by additional rinsing of reagent pipettors. Even with plasma exhibiting CK activities as high as 40.000 U/l, sample carryover by the c8000 could not be detected. Comparison of methods run on the c8000 and the Aeroset revealed correlation coefficients of 0.98-1.00; if identical chemistries were applied on both analyzers, slopes of regression lines approached unity. With typical laboratory workloads including 10-20% STAT samples and up to 10% samples with high analyte concentrations demanding dilutional reruns, steady-state throughput numbers of 700 to 800 tests per hour were obtained with the c8000. The system generally responded to STAT orders within 2 minutes yielding analytical STAT order completion times of 5 to 15 minutes depending on the type and number of assays requested per sample. Due to its extended test and sample processing capabilities and highly comfortable software, the c8000 may meet the varying needs of clinical laboratories rather well.
应用基本的电位分析法和光度分析法，我们评估了全自动随机存取化学分析仪Architect c8000（雅培Architect系统家族的新成员）的分析性能和操作性能，并将其与成熟的高通量化学平台雅培Aeroset进行了比较。我们的结果表明，c8000的批内和批间不精密度、不准确度、检测下限和线性范围总体上符合实验室诊断的实际要求；只有极少数例外情况，例如血浆脂肪酶或尿酸检测，显然需要通过额外冲洗试剂移液器来改进。即使血浆中的肌酸激酶（CK）活性高达40000 U/l，也未检测到c8000有样本残留。在c8000和Aeroset上运行的方法比较显示，相关系数为0.98 - 1.00；如果在两台分析仪上应用相同的化学方法，回归线的斜率接近1。对于包含10 - 20%急诊样本以及高达10%需要稀释重测的高分析物浓度样本的典型实验室工作量，使用c8000每小时可获得700至800次检测的稳态通量。该系统通常在2分钟内响应急诊指令，根据每个样本所需检测的类型和数量，分析急诊指令的完成时间为5至15分钟。由于其扩展的检测和样本处理能力以及高度便捷的软件，c8000可能很好地满足临床实验室的各种需求。
