亚洲腹泻儿童人群中腺病毒感染的分子流行病学
Molecular epidemiology of adenovirus infection among pediatric population with diarrhea in Asia.
作者信息
Li Lei, Phan Tung Gia, Nguyen Tuan Anh, Kim Kyo Sun, Seo Jeong Kee, Shimizu Hideaki, Suzuki Eiko, Okitsu Shoko, Ushijima Hiroshi
机构信息
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Japan.
出版信息
Microbiol Immunol. 2005;49(2):121-8. doi: 10.1111/j.1348-0421.2005.tb03711.x.
A total of 3,577 fecal specimens from infants and children with acute gastroenteritis in Japan, Korea and Vietnam during 1998 and 2001 were tested for adenovirus by the ELISA method. Of these, adenovirus was detected in 158 (4.4%). The detection rate of adenovirus was highest in Korea (8.7%, 20/231) followed by 5.0% (100/1,991) in Japan and 2.8% (38/1,355) in Vietnam. All adenoviruses were further serotyped by PCR-RFLP. The diversity of adenovirus serotypes including Ad2, 3, 5, 8, 31, 40 and 41 was demonstrated. Worth of note was a decrease in the rate of isolation of Ad40 (7.6%, 12/158) and a concomitant increase of Ad41 (63.9%, 101/158) to become the predominant serotype. Another interesting feature of the study was the presence of Ad3 (4.0% and 30%) and Ad8 (8.0% and 20%) in Japan and Korea, respectively, which is mainly associated with keratoconjunctivitis worldwide. Our result underscored the importance of adenovirus in association with acute gastroenteritis in Asian countries.
1998年至2001年期间，采用酶联免疫吸附测定法（ELISA）对日本、韩国和越南患有急性肠胃炎的婴幼儿和儿童的3577份粪便样本进行了腺病毒检测。其中，检测出腺病毒的样本有158份（4.4%）。腺病毒检出率在韩国最高（8.7%，20/231），其次是日本的5.0%（100/1991）和越南的2.8%（38/1355）。所有腺病毒均通过聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）进一步进行血清分型。结果显示包括Ad2、3、5、8、31、40和41在内的腺病毒血清型具有多样性。值得注意的是，Ad40的分离率有所下降（7.6%，12/158），而Ad41的分离率相应上升（63.9%，101/158），成为主要血清型。该研究的另一个有趣特征是，Ad3分别在日本（4.0%）和韩国（30%）检出，Ad8分别在日本（8.0%）和韩国（20%）检出，而在全球范围内，这两种血清型主要与角结膜炎相关。我们的研究结果强调了腺病毒在亚洲国家与急性肠胃炎的关联中的重要性。
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