Abe C, Hosojima S, Fukasawa Y, Kazumi Y, Takahashi M, Hirano K, Mori T
Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo.
J Clin Microbiol. 1992 Apr;30(4):878-81. doi: 10.1128/jcm.30.4.878-881.1992.
The rate of recovery and time to the detection of mycobacteria from clinical specimens were measured for biphasic (MB-Check; Nippon Roche Co., Ltd., Tokyo, Japan) and radiometric (BACTEC; Nippon Becton Dickinson Co., Ltd., Tokyo, Japan) liquid-based culture systems and egg-based media (3% Ogawa and Ogawa K). From the 245 sputum specimens processed, a total of 86 (35.1%) mycobacterial isolates were detected. Of these, 81 (94.2%) and 80 (93.0%) isolates were detected with the MB-Check and BACTEC systems, respectively, and 65 (75.6%) isolates were detected with the 3% Ogawa egg method. The difference in the percentages of positive cultures between the two systems based on liquid media and the 3% Ogawa egg method was significant (P less than 0.01). This difference was even greater among smear-negative specimens. The detection time was shorter with the liquid-based systems. The mean times to the detection of the Mycobacterium tuberculosis complex were 19.1 days with the MB-Check system, 13.4 days with the BACTEC system, and 21.7 days with the 3% Ogawa egg method. These results indicate that both the MB-Check and the BACTEC systems, based on liquid media, are efficient for the recovery of mycobacteria.
对双相（MB-Check；日本东京罗氏公司）和放射测量法（BACTEC；日本东京BD公司）液体培养系统以及基于鸡蛋的培养基（3%小川培养基和小川K培养基），测量了从临床标本中分离出分枝杆菌的回收率和检测时间。在处理的245份痰标本中，共检测到86株（35.1%）分枝杆菌分离株。其中，分别用MB-Check系统和BACTEC系统检测到81株（94.2%）和80株（93.0%）分离株，用3%小川鸡蛋培养基法检测到65株（75.6%）分离株。基于液体培养基的两种系统与3%小川鸡蛋培养基法之间阳性培养率的差异具有统计学意义（P小于0.01）。这种差异在涂片阴性标本中更大。基于液体的系统检测时间更短。检测结核分枝杆菌复合群的平均时间，MB-Check系统为19.1天，BACTEC系统为13.4天，3%小川鸡蛋培养基法为21.7天。这些结果表明，基于液体培养基的MB-Check系统和BACTEC系统在分枝杆菌的回收方面都是有效的。
