在存在血清抑制剂的情况下，不同方法检测甲型流感病毒特异性血凝抑制抗体的比较。
Comparison of different approaches to measuring influenza A virus-specific hemagglutination inhibition antibodies in the presence of serum inhibitors.
作者信息
Subbarao E K, Kawaoka Y, Ryan-Poirier K, Clements M L, Murphy B R
机构信息
Respiratory Viruses Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
出版信息
J Clin Microbiol. 1992 Apr;30(4):996-9. doi: 10.1128/jcm.30.4.996-999.1992.
Abstract
The A/Los Angeles/2/87 (H3N2) (A/LA/2/87) virus is sensitive to inhibitors of hemagglutination present in certain human sera. It was found that the effect of these inhibitors could be removed by treating sera with high-concentration receptor-destroying enzyme or trypsin-periodate or by using inhibitor-resistant viruses in the hemagglutination inhibition (HAI) test. Inhibitor-resistant viruses were not effective for detecting rises in antibody titers in the sera of volunteers infected with the A/LA/2/87 wild-type virus, while rises in antibody titer were readily detected in sera treated with trypsin-periodate and tested against A/LA/2/87 wild-type virus in an HAI test. It is therefore suggested that chemical or enzymatic methods be used to inactivate serum inhibitors and that standard virus be used in the HAI test for the currently circulating H3N2 viruses.
摘要
A/洛杉矶/2/87（H3N2）（A/LA/2/87）病毒对某些人血清中存在的血凝抑制物敏感。研究发现，通过用高浓度受体破坏酶或胰蛋白酶-高碘酸盐处理血清，或在血凝抑制（HAI）试验中使用抗抑制剂病毒，可以消除这些抑制剂的作用。抗抑制剂病毒在检测感染A/LA/2/87野生型病毒的志愿者血清中抗体滴度的升高方面无效，而在用胰蛋白酶-高碘酸盐处理并在HAI试验中针对A/LA/2/87野生型病毒进行检测的血清中，很容易检测到抗体滴度的升高。因此，建议使用化学或酶促方法使血清抑制剂失活，并在HAI试验中使用标准病毒检测当前流行的H3N2病毒。
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