Yanase Hideshi, Nozaki Koji, Okamoto Kenji
Department of Biotechnology, Tottori University, Tottori, Tottori, 680-8552, Japan.
Biotechnol Lett. 2005 Feb;27(4):259-63. doi: 10.1007/s10529-004-8295-1.
To confer the ability to ferment cello-oligosaccharides on the ethanol-producing bacterium, Zymomonas mobilis, the beta-glucosidase gene from Ruminococcus albus, tagged at its N-terminal with the 53-amino acid Tat signal peptide from the periplasmic enzyme glucose-fructose oxidoreductase from Z. mobilis, was introduced into the strain. The tag enabled 61% of the beta-glucosidase activity to be transported through the cytoplasmic membrane of the recombinant strain which then produced 0.49 g ethanol/g cellobiose.
为了赋予产乙醇细菌运动发酵单胞菌发酵纤维二糖的能力，将来自白色瘤胃球菌的β-葡萄糖苷酶基因导入该菌株，该基因在其N端标记了来自运动发酵单胞菌周质酶葡萄糖-果糖氧化还原酶的53个氨基酸的Tat信号肽。该标签使61%的β-葡萄糖苷酶活性能够通过重组菌株的细胞质膜运输，该重组菌株随后每克纤维二糖产生0.49克乙醇。
