Lee Myoung Woo, Yang Mal Sook, Park Joon Seong, Kim Hugh C, Kim Young Jin, Choi Jeongeun
Biomedical Research Institute, LifeCord Inc, Suwon, Korea.
Int J Hematol. 2005 Feb;81(2):126-30. doi: 10.1532/ijh97.a10404.
Umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages. Because mesenchymal stem cells (MSCs) from bone marrow have been regarded as good materials for cell/gene therapy as well as for tissue engineering because of their multidifferentiation potential, a number of trials have been undertaken to isolate MSCs from UCB. However, the results have been controversial, and little has become known about the effect of cryopreservation on the isolation of these stem cells. In this study, we examined the ability of cryopreserved UCB-derived cells to produce MSCs. Various culture conditions, including the seeding concentrations of cells and the media used, were investigated. We were able to obtain adherent cell populations after 3 to 5 weeks in our culture conditions from UCB-derived mononuclear cell fractions that had undergone cryopreservation for 0.1 to 5 years. These cells exhibited a fibroblast-like morphology and typical mesenchymal-like immunophenotypes. The results indicate that cryopreserved human UCB fractions can be used as an alternative source of MSCs for experimental and clinical applications as well as for tissue engineering.
脐带血（UCB）是众所周知的丰富造血干细胞来源，具有实际和伦理优势。由于骨髓间充质干细胞（MSCs）因其多向分化潜能而被视为细胞/基因治疗以及组织工程的良好材料，因此已经进行了许多试验以从脐带血中分离间充质干细胞。然而，结果一直存在争议，而且关于冷冻保存对这些干细胞分离的影响知之甚少。在本研究中，我们检测了冷冻保存的脐带血来源细胞产生间充质干细胞的能力。研究了各种培养条件，包括细胞接种浓度和所用培养基。在我们的培养条件下，从经过0.1至5年冷冻保存的脐带血来源单核细胞组分中，3至5周后我们能够获得贴壁细胞群。这些细胞呈现成纤维细胞样形态和典型的间充质样免疫表型。结果表明，冷冻保存的人脐带血组分可作为间充质干细胞的替代来源，用于实验和临床应用以及组织工程。
