Olivero A, Miglietta A, Gadoni E, Gabriel L
Dipartimento di Medicina ed Oncologia Sperimentale, Sezione di Patologia Generale, Torino, Italy.
Cell Biochem Funct. 1990 Apr;8(2):99-105. doi: 10.1002/cbf.290080204.
4-Hydroxynonenal, which is one of the most important products of lipid peroxidation, alters microtubular organization and structure in 3T3 fibroblasts. Changes in cell shape and the disappearance of microtubules are observed by immunofluorescence after incubation with the aldehyde, and the colchicine binding activity of tubulin from 3T3 cells is modified. Moreover, the aldehyde determines a decrease in the ability of purified tubulin to polymerize and to bind colchicine. These effects may be related to the interaction of the aldehyde with functional -SH groups of tubulin which are necessary for protein integrity and functions. Indeed, the addition of cysteine protects against the damaging effects of the aldehyde.
4-羟基壬烯醛是脂质过氧化作用最重要的产物之一，它会改变3T3成纤维细胞中的微管组织和结构。在用该醛孵育后，通过免疫荧光观察到细胞形态的变化和微管的消失，并且3T3细胞微管蛋白的秋水仙碱结合活性发生了改变。此外，该醛导致纯化的微管蛋白聚合及结合秋水仙碱的能力下降。这些效应可能与醛和微管蛋白的功能性巯基相互作用有关，而这些巯基对于蛋白质的完整性和功能是必需的。事实上，添加半胱氨酸可防止醛的损伤作用。
