Miglietta A, Gabriel L
Res Commun Chem Pathol Pharmacol. 1986 Feb;51(2):245-60.
Methylglyoxal antiproliferative action is well known; this action may be exerted by a non-enzymic mechanism and may directly involve the microtubular system. Our purpose was to verify this hypothesis, by studying the aldehyde effect on normal and tumour microtubular protein. Methylglyoxal incubation of normal and tumour liver cell homogenates causes inhibition of colchicine binding activity above all at higher concentrations. The aldehyde administration to the purified microtubular protein induces alterations in some tubulin properties already at lower concentrations. These results show a possible tubulin-methylglyoxal interaction, also if the reaction mechanism is so far unclear.
甲基乙二醛的抗增殖作用是众所周知的；这种作用可能通过非酶机制发挥，并且可能直接涉及微管系统。我们的目的是通过研究醛对正常和肿瘤微管蛋白的影响来验证这一假设。正常和肿瘤肝细胞匀浆与甲基乙二醛孵育会导致秋水仙碱结合活性受到抑制，尤其是在较高浓度时。向纯化的微管蛋白中加入醛，即使在较低浓度下也会引起一些微管蛋白性质的改变。这些结果表明微管蛋白与甲基乙二醛之间可能存在相互作用，尽管到目前为止反应机制尚不清楚。
