Sullivan Christopher B, Diggle Matthew A, Clarke Stuart C
Scottish Meningococcus and Pneumococcus Reference Laboratory North Glasgow University Hospital NHS Trust, Glasgow, UK.
Mol Biotechnol. 2005 Mar;29(3):245-54. doi: 10.1385/MB:29:3:245.
Numerous computer-based statistical packages have been developed in recent years and it has become easier to analyze nucleotide sequence data and gather subsequent information that would not normally be available. Multilocus sequence typing (MLST) is used for characterizing isolates of bacterial and fungal species and uses nucleotide sequences of internal fragments of housekeeping genes. This method is finding a place in clinical microbiology and public health by providing data for epidemiological surveillance and development of vaccine policy. It adds greatly to our knowledge of the genetic variation that can occur within a species and has therefore been used for studies of population biology. Analysis requires the detailed interpretation of nucleotide sequence data obtained from housekeeping and nonhousekeeping genes. This is due to the amount of data generated from nucleotide sequencing and the information generated from an array of analytical tools improves our understanding of bacterial pathogens. This can benefit public health interventions and the development of enhanced therapies and vaccines. This review concentrates on the analytical tools used in MLST and their use in the clinical microbiology and public health fields.
近年来，已经开发出了许多基于计算机的统计软件包，分析核苷酸序列数据并收集通常无法获得的后续信息变得更加容易。多位点序列分型（MLST）用于鉴定细菌和真菌物种的分离株，并使用管家基因内部片段的核苷酸序列。通过提供用于流行病学监测和疫苗政策制定的数据，这种方法在临床微生物学和公共卫生领域正占据一席之地。它极大地增加了我们对一个物种内可能发生的遗传变异的了解，因此已被用于种群生物学研究。分析需要对从管家基因和非管家基因获得的核苷酸序列数据进行详细解读。这是因为核苷酸测序产生的数据量以及一系列分析工具产生的信息提高了我们对细菌病原体的理解。这可以使公共卫生干预措施以及增强型疗法和疫苗的开发受益。本综述集中讨论了MLST中使用的分析工具及其在临床微生物学和公共卫生领域的应用。
