具有来自编码牛分枝杆菌卡介苗蛋白质的cDNA序列的合成寡核苷酸的抗肿瘤活性。
Antitumor activity of synthetic oligonucleotides with sequences from cDNA encoding proteins of Mycobacterium bovis BCG.
作者信息
Kataoka T, Yamamoto S, Yamamoto T, Kuramoto E, Kimura Y, Yano O, Tokunaga T
机构信息
Department of Cellular Immunology, National Institute of Health, Tokyo.
出版信息
Jpn J Cancer Res. 1992 Mar;83(3):244-7. doi: 10.1111/j.1349-7006.1992.tb00094.x.
Thirteen kinds of 45-mer or 30-mer synthetic oligonucleotides with sequences randomly selected from the cDNA encoding three kinds of protein of Mycobacterium bovis BCG were tested for their antitumor activity in a murine tumor system. Six out of the 13 single-stranded oligonucleotides which contained one or more hexameric palindromic sequences showed strong antitumor activity while the others without palindromic structure did not. Namely, repeated intralesional injections of 100 micrograms of the 6 oligonucleotides caused regression of the established tumor but the other 7 were ineffective. When tumor cells were mixed with 100 micrograms of an effective oligonucleotide and injected into mice, tumor growth was markedly suppressed. These results suggested that palindromic structure is essential for the antitumor activity of the synthetic oligonucleotides.
从编码牛分枝杆菌卡介苗三种蛋白质的cDNA中随机选择序列，合成了13种45聚体或30聚体的寡核苷酸，并在小鼠肿瘤系统中测试了它们的抗肿瘤活性。13种单链寡核苷酸中有6种含有一个或多个六聚体回文序列，表现出很强的抗肿瘤活性，而其他没有回文结构的则没有活性。也就是说，在瘤内反复注射100微克这6种寡核苷酸可使已形成的肿瘤消退，但另外7种则无效。当将肿瘤细胞与100微克有效的寡核苷酸混合后注射到小鼠体内时，肿瘤生长受到明显抑制。这些结果表明，回文结构对于合成寡核苷酸的抗肿瘤活性至关重要。
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