Lin Shih-Shen, Chou Ming-Yung, Ho Chuan-Chen, Kao Chia-Tze, Tsai Chung-Hung, Wang Lina, Yang Chi-Chiang
Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC.
Microbes Infect. 2005 Apr;7(4):635-44. doi: 10.1016/j.micinf.2004.12.023. Epub 2005 Mar 18.
Mouth ulcers are one of the most common oral complaints. However, the association between oral ulceration and viruses and cytokines is uncertain. We detected the presence of human papilloma virus (HPV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus (HSV)-1, HSV-2 and human herpesvirus (HHV)-8 DNA in oral tissues by polymerase chain reaction (PCR) and Southern hybridization techniques, and quantified the serum levels of cytokines including interleukin (IL)-2, IL-4, granulocyte-macrophage colony-stimulating factor (GM-CSF), soluble Fas (sFas) and the Fas ligand (FasL) by enzyme-linked immunosorbent assay (ELISA) for 67 recurrent aphthous ulcer (RAU) patients and 72 normal individuals. Seven patient specimens were excluded from the study due to the negative PCR results for the beta-globin used as the internal control. Among the 32 (53.3%) virus-positive results from 60 patients' samples, 8 (13.3%) HPV, 4 (6.7%) HSV-1, 11 (18.3%) CMV, 9 (15.0%) EBV, and 16 (26.7%) HHV-8 samples proved to be positive. No HSV-2-positive samples were found. The percentage of single-virus infection (56.3%) was significantly greater than that of double-virus co-infection (31.3%) and the percentage of double-virus co-infection was significantly greater than the percentage of triple-virus co-infection (12.5%) (P < 0.05). In the 72 normal oral-tissue specimens, no viral DNA was detected. The mean serum cytokine level for patients was significantly (P < 0.05) greater than for controls for most of the separate age groups. The mean serum cytokine concentrations for the patient group demonstrated a diffuse pattern covering a wide range of serum concentrations, a very different result from the compact serum concentration pattern and lower mean serum cytokine concentrations revealed by the normal group. Overall association between viruses and recurrent aphthous ulceration is HHV-8 > CMV > EBV > HPV > HSV-1, regarding the frequency of prevalence (P < 0.05).
口腔溃疡是最常见的口腔疾病之一。然而，口腔溃疡与病毒及细胞因子之间的关联尚不确定。我们采用聚合酶链反应（PCR）和Southern杂交技术检测口腔组织中人类乳头瘤病毒（HPV）、巨细胞病毒（CMV）、EB病毒（EBV）、单纯疱疹病毒1型（HSV-1）、单纯疱疹病毒2型（HSV-2）和人类疱疹病毒8型（HHV-8）DNA的存在情况，并通过酶联免疫吸附测定（ELISA）对67例复发性阿弗他溃疡（RAU）患者和72例正常个体血清中包括白细胞介素（IL）-2、IL-4、粒细胞巨噬细胞集落刺激因子（GM-CSF）、可溶性Fas（sFas）和Fas配体（FasL）等细胞因子水平进行定量分析。由于作为内对照的β-珠蛋白PCR结果为阴性，7例患者标本被排除在研究之外。在60例患者样本中的32例（53.3%）病毒阳性结果中：8例（13.3%）HPV、4例（6.7%）HSV-1、11例（18.3%）CMV、9例（15.0%）EBV和16例（26.7%）HHV-8样本被证实为阳性。未发现HSV-2阳性样本。单病毒感染的百分比（56.3%）显著高于双病毒合并感染的百分比（31.3%），双病毒合并感染的百分比显著高于三病毒合并感染的百分比（12.5%）（P<0.05）。在72例正常口腔组织标本中，未检测到病毒DNA。在大多数不同年龄组中，患者的平均血清细胞因子水平显著高于对照组（P<0.05）。患者组的平均血清细胞因子浓度呈现出一种覆盖广泛血清浓度范围的弥散模式，这与正常组呈现出的紧凑血清浓度模式及较低的平均血清细胞因子浓度截然不同。就患病率而言，病毒与复发性阿弗他溃疡之间的总体关联为HHV-8>CMV>EBV>HPV>HSV-1（P<0.05）。
