Sargent T D, Wu J R, Sala-Trepat J M, Wallace R B, Reyes A A, Bonner J
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60. doi: 10.1073/pnas.76.7.3256.
The rat serum albumin gene has been isolated from a recombinant library containing the entire rat genome cloned in the lambda phage Charon 4A. Preliminary R-loop and restriction analysis has revealed that this gene is split into at least 14 fragments (exons) by 13 intervening sequences (introns), and that it occupies a minimum of 14.5 kilobases of genomic DNA.
大鼠血清白蛋白基因已从一个重组文库中分离出来,该文库包含克隆在λ噬菌体Charon 4A中的完整大鼠基因组。初步的R环和限制性分析表明,该基因被13个间隔序列(内含子)分成至少14个片段(外显子),并且它占据至少14.5千碱基的基因组DNA。