Allan R N, Lebbe L, Heyrman J, De Vos P, Buchanan C J, Logan N A
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
Vakgroep BFM WE 10V Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1039-1050. doi: 10.1099/ijs.0.63397-0.
Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that L-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for L-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K(+) antiport system and similar energy systems for the uptake of l-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657(T) (= LMG 22481(T) = CIP 108307(T)) and Logan B-1599(T) (LMG 22483(T) = CIP 108308(T)), respectively.
从隐花植物岭、墨尔本山北坡的地热土壤以及南极维多利亚地北部里特曼山的喷口和山顶分离出了13株产芽孢细菌。16S rRNA基因测序、SDS - PAGE和常规表型特征测试表明，从墨尔本山北坡分离出的7株菌株代表了短芽孢杆菌属的一个新物种，而从隐花植物岭以及里特曼山的喷口和山顶分离出的6株菌株代表了食酸菌属的一个新物种。在里特曼山或隐花植物岭的采样点未分离到短芽孢杆菌属菌株，在墨尔本山北坡也未分离到食酸菌属菌株。初步代谢研究表明，L - 谷氨酸虽然不是生长所必需的，但这两个物种都能利用它。短芽孢杆菌属物种拥有一个对L - 谷氨酸特异的摄取系统，而食酸菌属物种拥有一个更通用的摄取系统，能够转运其他相关氨基酸。这两个物种都利用K(+)反向转运系统和类似的能量系统来摄取L - 谷氨酸。短芽孢杆菌属物种模式菌株的摄取速率比食酸菌属物种模式菌株快20倍。提议将新分类群命名为新种利氏短芽孢杆菌（Brevibacillus levickii sp. nov.）和新种新地食酸菌（Aneurinibacillus terranovensis sp. nov.）；模式菌株分别为Logan B - 1657(T)（= LMG 22481(T) = CIP 108307(T)）和Logan B - 1599(T)（LMG 22483(T) = CIP 108308(T)）。
