稻多形单胞菌属，新属，新种，一种从水稻稻田土壤中分离出的固氮细菌。
Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa.
作者信息
Xie Cheng-Hui, Yokota Akira
机构信息
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
出版信息
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1233-1237. doi: 10.1099/ijs.0.63406-0.
Five strains of free-living diazotrophs isolated from rice were characterized by using a polyphasic approach. The strains were found to be very closely related, with 99-100 % 16S rRNA gene sequence similarity and DNA-DNA hybridization values greater than 70 %, suggesting that they represent a single species. When compared with other recognized species, they showed not more than 93 and 89 % similarity for the 16S rRNA and nifH gene sequences, respectively. Phylogenetic distances showed that these isolates were distinct from other taxa within the alpha-Proteobacteria. Chemotaxonomic characteristics of these isolates included the DNA G + C content (62.1-63.1 mol%), the major quinone system (Q-10), predominant fatty acids (18 : 1omega7c, cyclo 19 : 0omega8c and 16 : 0) and major hydroxy fatty acids (14 : 0 3-OH, 18 : 0 3-OH and 16 : 0 3-OH). Based on phylogenetic and phenotypic analyses, these isolates are considered to represent a novel genus and species, for which the name Pleomorphomonas oryzae gen. nov., sp. nov. is proposed. The type strain is F-7(T) (=IAM 15079(T) = ATCC BAA-940(T) = DSM 16300(T)).
采用多相分类法对从水稻中分离出的5株自生固氮菌进行了特征分析。结果发现这些菌株之间亲缘关系非常密切，16S rRNA基因序列相似性为99 - 100%，DNA - DNA杂交值大于70%，表明它们代表一个单一物种。与其他已确认的物种相比，它们的16S rRNA和nifH基因序列相似性分别不超过93%和89%。系统发育距离表明这些分离株与α-变形菌纲内的其他分类单元不同。这些分离株的化学分类特征包括DNA G + C含量（62.1 - 63.1 mol%）、主要醌系统（Q - 10）、主要脂肪酸（18 : 1ω7c、环丙烷19 : 0ω8c和16 : 0）以及主要羟基脂肪酸（14 : 0 3 - OH、18 : 0 3 - OH和16 : 0 3 - OH）。基于系统发育和表型分析，这些分离株被认为代表一个新的属和种，为此提出新属名为Pleomorphomonas，新种名为oryzae gen. nov., sp. nov.。模式菌株为F - 7(T)（=IAM 15079(T) = ATCC BAA - 940(T) = DSM 16300(T)）。
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