Kato Takeshi, Ohno Osamu, Nagoshi Toshimasa, Ichinose Yoshihiro, Igarashi Shukuro
Department of Materials Science, Faculty of Engineering, Ibaraki University, Nakanarusawa 4-12-1, Hitachi, Ibaraki 316-8511, Japan.
Anal Sci. 2005 May;21(5):579-81. doi: 10.2116/analsci.21.579.
Chemiluminescence (CL) was immediately observed after an iron-chlorophyllin aqueous solution was added to an acetonitrile/water mixed solution containing hydrogen peroxide. Quenching of the iron-chlorophyllin complex CL was caused by adding L-ascorbic acid. Based on these facts, a determination method involving small amounts of L-ascorbic acid was developed. As a result, this CL system is able to determine L-ascorbic acid over a wide concentration range of 4.0 x 10(-12) to 4.0 x 10(-4) mol L(-1). Also, coexisting substances, such as sugar and vitamins, did not interfere with the determination. Moreover, the participation to the CL was not observed when using other reducing agents, such as hydroxylamine hydrochloride. As an application for practical use, L-ascorbic acid in soft-drink powder was determined. The experimental value was almost the same as the calculated one (5.30 x 10(-5) mol L(-1)).
将叶绿素铁水溶液加入到含有过氧化氢的乙腈/水混合溶液中后，立即观察到化学发光（CL）现象。加入L-抗坏血酸会导致叶绿素铁络合物的CL猝灭。基于这些事实，开发了一种测定少量L-抗坏血酸的方法。结果，该CL系统能够在4.0×10⁻¹²至4.0×10⁻⁴mol L⁻¹的宽浓度范围内测定L-抗坏血酸。此外，糖和维生素等共存物质不会干扰测定。而且，使用其他还原剂（如盐酸羟胺）时未观察到对CL的参与。作为实际应用，测定了软饮料粉末中的L-抗坏血酸。实验值与计算值（5.30×10⁻⁵mol L⁻¹）几乎相同。
