Liebregts T, Adam B, Bertel A, Jones S, Schulze J, Enders C, Sonnenborn U, Lackner K, Holtmann G
Department of Gastroenterology, Hepatology and General Medicine, University of Adelaide, Royal Adelaide Hospital, South Australia, Adelaide, Australia.
Neurogastroenterol Motil. 2005 Jun;17(3):410-4. doi: 10.1111/j.1365-2982.2005.00638.x.
Visceral hyperalgesia (VH) plays a key role for the manifestation of functional gastrointestinal (GI) disorders. In a subgroup of patients, the initial manifestation is preceded by GI inflammation. Recent studies have demonstrated an improvement of inflammation and symptoms during treatment with Escherichia coli Nissle 1917 (EcN).
We aimed to characterize the effects of EcN on visceral sensitivity in a rat model of post-inflammatory VH.
Male Lewis rats underwent colorectal instillation of trinitrobenzenesulphonic acid (TNBS) plus an equal amount of ethanol (test group) or physiological saline solution (control group). After 28, 35 and 42 days, standardized colorectal distensions were performed and the visceromotor reflex (VMR) of abdominal wall muscles was quantified by electromyographic recording. From day 28 onwards, EcN was administered in drinking water.
After TNBS, a significant increase of VMR was observed compared with saline controls over all study days. Administration of EcN reduced the TNBS-induced hyperalgesia [EcN: 863+/-125 microV vs placebo: 1258+/-157 microV (P<0.05)] at day 35, while there were no significant alterations at any other study day.
The EcN administration caused a significant reduction of VH. Whether EcN might play a role in the treatment of post-infectious functional bowel disorders remains to be investigated in further studies.
内脏高敏在功能性胃肠疾病的表现中起关键作用。在一部分患者中，最初的表现之前有胃肠道炎症。最近的研究表明，在用大肠杆菌Nissle 1917（EcN）治疗期间，炎症和症状有所改善。
我们旨在表征EcN对炎症后内脏高敏大鼠模型中内脏敏感性的影响。
雄性Lewis大鼠接受三硝基苯磺酸（TNBS）加等量乙醇的结肠内灌注（试验组）或生理盐水（对照组）。在28、35和42天后，进行标准化的结肠扩张，并通过肌电图记录量化腹壁肌肉的内脏运动反射（VMR）。从第28天起，在饮用水中给予EcN。
与生理盐水对照组相比，在所有研究天数中，TNBS后观察到VMR显著增加。在第35天，给予EcN可减轻TNBS诱导的痛觉过敏[EcN：863±125微伏，安慰剂：1258±157微伏（P<0.05）]，而在任何其他研究天数均无显著变化。
给予EcN可显著减轻内脏高敏。EcN是否可能在感染后功能性肠病的治疗中发挥作用，仍有待进一步研究。
