Siqueira J F, Rôças I N
Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil.
J Endod. 2005 Jun;31(6):411-23. doi: 10.1097/01.don.0000157989.44949.26.
Endodontic infections have been traditionally studied by culture-dependent methods. However, as with other areas of clinical microbiology, culture-based investigations are plagued by significant problems, including the probable involvement of viable but uncultivable micro-organisms with disease causation and inaccurate microbial identification. Innumerous molecular technologies have been used for microbiological diagnosis in clinical microbiology, but only recently some of these techniques have been applied in endodontic microbiology research. This paper intended to review the main molecular methods that have been used or have the potential to be used in the study of endodontic infections. Moreover, advantages and limitations of current molecular techniques when compared to conventional methods for microbial identification are also discussed.
传统上，牙髓感染是通过依赖培养的方法进行研究的。然而，与临床微生物学的其他领域一样，基于培养的研究存在重大问题，包括可能存在的活的但不可培养的微生物与疾病病因的关联以及微生物鉴定不准确。临床微生物学中已使用了无数种分子技术进行微生物诊断，但直到最近其中一些技术才应用于牙髓微生物学研究。本文旨在综述已用于或有可能用于牙髓感染研究的主要分子方法。此外，还讨论了当前分子技术与传统微生物鉴定方法相比的优点和局限性。
