Jaramillo-Juárez F, Rodríguez-Vázquez M L, Muñoz-Martínez J, Quezada-Tristán T, Posadas del Río F A, Llamas-Viramontes J, Ortíz G Gabriel, Feria-Velasco Alfredo, Reyes J L
Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Avenida Universidad 940, Fraccionamiento Los Bosques, CP 20100 Aguascalientes, Ags., Mexico.
Toxicon. 2005 Jul;46(1):99-103. doi: 10.1016/j.toxicon.2005.03.019.
Our previous acute toxicity studies with Karwinskia humboldtiana (Kh) in rats showed renal hemodynamic changes with a marked increase in the fractional excretion of sodium and morphological damage. To analyse the effects of Kh or 'tullidora' on energetic metabolism, a single dose of an oral preparation from the seed fruits was given to Wistar rats (1.25 g/kg). In tullidora-treated rats there was 8% mortality. ATP concentrations in renal tissue decreased significantly (control: 53.85+/-3.34, tullidora 38.28+/-5.31 micromol/g fresh tissue, P<0.05). Total blood (54.8+/-0.96, tullidora: 40.2+/-1.55 micromol/dL, P<0.01) and haemoglobin-ATP concentrations (3.69+/-0.12, tullidora: 2.56+/-0.11 micromol/g, P<0.01) were also significantly diminished. Moreover, the total protein in renal cortex from tullidora-treated rats decreased as compared to control group (control: 71.43+/-2.88, tullidora: 55.20+/-4.06 mg/g fresh tissue, P<0.05). In contrast, Na+-K+-ATPase activity in tullidora-treated animals was not different from control rats. These findings might partially explain the acute effects and mortality observed in the Kh treated rats.
我们之前对大鼠进行的关于 Humboldt 氏卡氏藤（Kh）的急性毒性研究表明，其会引起肾脏血流动力学变化，钠排泄分数显著增加以及形态学损伤。为了分析 Kh 或“tullidora”对能量代谢的影响，给 Wistar 大鼠口服一剂种子果实的制剂（1.25 g/kg）。在接受 tullidora 治疗的大鼠中，死亡率为 8%。肾组织中的 ATP 浓度显著降低（对照组：53.85±3.34，tullidora 组：38.28±5.31 μmol/g 新鲜组织，P<0.05）。全血（对照组：54.8±0.96，tullidora 组：40.2±1.55 μmol/dL，P<0.01）和血红蛋白-ATP 浓度（对照组：3.69±0.12，tullidora 组：2.56±0.11 μmol/g，P<0.01）也显著降低。此外，与对照组相比，接受 tullidora 治疗的大鼠肾皮质中的总蛋白减少（对照组：71.43±2.88，tullidora 组：55.20±4.06 mg/g 新鲜组织，P<0.05）。相比之下，接受 tullidora 治疗的动物的 Na+-K+-ATP 酶活性与对照大鼠没有差异。这些发现可能部分解释了在接受 Kh 治疗的大鼠中观察到的急性效应和死亡率。
