Flores-Otero G, Cueva J, Muñoz-Martínez E J, Rubio-Franchini C
Toxicon. 1987;25(4):419-26. doi: 10.1016/0041-0101(87)90075-4.
The absorbance spectrum of tullidinol, a tullidora (Karwinskia humboldtiana) toxin, showed peaks at 260 and 410 nm when dissolved in ethanol; the second peak was linearly related to toxin concentration. Silica gel thin layer chromatograms of tullidinol solutions showed a single, yellow spot with an Rf of 0.76. A single dose of tullidora fruit homogenate was orally given to male Wistar rats; the absorbance spectrum of serum from tullidora-treated rats showed peaks at 300 nm and at 410 nm; the second peak was absent in serum from control rats. No spots were detected in chromatograms of normal serum, but a yellow spot with the same Rf as that of tullidinol was found in the serum of tullidora-treated rats. We conclude that tullidinol was detected in the serum of treated animals and that the methods we used for its detection may also help to diagnose accidental tullidora poisoning in cattle and human beings.
图利多醇是一种图利多拉（洪堡卡氏木）毒素，其在乙醇中溶解时的吸收光谱在260纳米和410纳米处有峰值；第二个峰值与毒素浓度呈线性相关。图利多醇溶液的硅胶薄层色谱图显示有一个单一的黄色斑点，比移值为0.76。给雄性Wistar大鼠口服单剂量的图利多拉果实匀浆；经图利多拉处理的大鼠血清的吸收光谱在300纳米和410纳米处有峰值；对照大鼠的血清中没有第二个峰值。正常血清的色谱图中未检测到斑点，但在经图利多拉处理的大鼠血清中发现了一个与图利多醇比移值相同的黄色斑点。我们得出结论，在经处理动物的血清中检测到了图利多醇，并且我们用于检测它的方法可能也有助于诊断牛和人类的意外图利多拉中毒。
