Sung Y C, Fuchs J A
Department of Biochemistry, University of Minnesota, St. Paul 55108.
J Bacteriol. 1992 Jun;174(11):3645-50. doi: 10.1128/jb.174.11.3645-3650.1992.
A regulatory gene, cynR, was found to be located next to the cyn operon but transcribed in the opposite direction. cynR encodes a positive regulatory protein that controls the cyn operon as well as its own synthesis. Positive regulation of the cyn operon requires cyanate and the cynR protein, but the negative autoregulation of the cynR gene appears to be independent of cyanate. The predicted amino acid sequence of the cynR protein derived from the DNA sequence was found to have significant homology to the predicted amino acid sequence of the lysR family of regulatory proteins.
发现一个调控基因cynR位于cyn操纵子旁边，但转录方向相反。cynR编码一种正向调控蛋白，该蛋白控制cyn操纵子及其自身的合成。cyn操纵子的正向调控需要氰酸盐和cynR蛋白，但cynR基因的负向自我调控似乎与氰酸盐无关。从DNA序列推导的cynR蛋白的预测氨基酸序列与调控蛋白lysR家族的预测氨基酸序列具有显著同源性。
