Yan Xun-Ling, Dong Rui-Xin, Zhang Lei, Zhang Xue-Jun, Zhang Zong-Wang
School of Physics Science and Information Engineering, Liaocheng University, Liaocheng 252059, Shandong Province, China.
World J Gastroenterol. 2005 Jun 7;11(21):3290-2. doi: 10.3748/wjg.v11.i21.3290.
To explore the difference between cancer cells and normal cells, we investigated the Raman spectra of single cells from gastrointestinal cancer patients.
All samples were obtained from 30 diagnosed as gastrointestinal cancer patients. The flesh tumor specimen is located in the center of tumor tissue, while the normal ones were 5 cm away from the outside tumor section. The imprint was put under the micros-cope and a single cell was chosen for Raman measurement. All spectra were collected at confocal Raman micro-spectroscopy (British Renishaw) with NIR 780 nm laser.
We measured the Raman spectra of several cells from gastrointestinal cancer patients. The result shows that there exists the strong line at 1 002 /cm with less half-width assigned to the phenylalanine in several cells. The Raman lines of white cell were lower and less, while those of red cell were not only higher in intensity and more abundant, but also had a particular C-N breathing stretching band of pyrrole ring at 1 620-1 540 /cm. The line at 1 084 /cm assigned to phosphate backbone of DNA became obviously weaker in cancer cell. The Raman spectra of stomach cancer cells were similar to those of normal cells, but the Raman intensity of cancer cells was much lower than that of normal cells, and even some lines disappear. The lines of enteric cancer cells became weaker than spectra above and many lines disappeared, and the cancer cells in different position had different fluorescence intensity.
The Raman spectra of several cells from cancer patients show that the structural changes of cancer cells happen and many bonds rupture so that the biological function of cells are lost. The results indicate that Raman spectra can offer the experiment basis for the cancer diagnosis and treatment.
为探究癌细胞与正常细胞之间的差异，我们对胃肠道癌患者的单细胞拉曼光谱进行了研究。
所有样本均取自30例确诊为胃肠道癌的患者。肿瘤组织样本取自肿瘤组织中心，正常组织样本则取自距肿瘤边缘5厘米处。将印记置于显微镜下，选取单个细胞进行拉曼测量。所有光谱均在配备近红外780纳米激光的共焦拉曼显微镜（英国雷尼绍公司）上采集。
我们测量了胃肠道癌患者多个细胞的拉曼光谱。结果显示，部分细胞中存在半高宽较窄的位于1002/cm处的强峰，归属于苯丙氨酸。白细胞的拉曼峰较低且较少，而红细胞的拉曼峰不仅强度更高、更为丰富，还在1620 - 1540/cm处有吡咯环的特定C - N呼吸伸缩带。归属于DNA磷酸骨架的1084/cm处的峰在癌细胞中明显减弱。胃癌细胞的拉曼光谱与正常细胞相似，但癌细胞的拉曼强度远低于正常细胞，甚至一些峰消失。肠道癌细胞的峰比上述光谱更弱，许多峰消失，且不同位置的癌细胞具有不同的荧光强度。
癌症患者多个细胞的拉曼光谱表明，癌细胞发生了结构变化，许多化学键断裂，导致细胞生物学功能丧失。结果表明，拉曼光谱可为癌症诊断和治疗提供实验依据。
