用于严重急性呼吸综合征血清学诊断的免疫荧光测定法。
Immunofluorescence assay for serologic diagnosis of SARS.
作者信息
Chan Paul K S, Ng King-Cheung, Chan Rickjason C W, Lam Rebecca K Y, Chow Viola C Y, Hui Mamie, Wu Alan, Lee Nelson, Yap Florence H Y, Cheng Frankie W T, Sung Joseph J Y, Tam John S
机构信息
Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China.
出版信息
Emerg Infect Dis. 2004 Mar;10(3):530-2. doi: 10.3201/eid1003.030493.
Abstract
We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
摘要
我们评估了一种基于病毒感染细胞的间接免疫荧光试验，用于检测抗严重急性呼吸综合征相关冠状病毒（SARS-CoV）免疫球蛋白（Ig）G抗体。所有确诊的SARS病例均显示血清转化或IgG抗体滴度呈四倍升高；无对照呈阳性。该试验的敏感性和特异性均为100%。免疫荧光试验可确定SARS-CoV感染状况。
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