Chan Paul K S, Ng King-Cheung, Chan Rickjason C W, Lam Rebecca K Y, Chow Viola C Y, Hui Mamie, Wu Alan, Lee Nelson, Yap Florence H Y, Cheng Frankie W T, Sung Joseph J Y, Tam John S
Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China.
Emerg Infect Dis. 2004 Mar;10(3):530-2. doi: 10.3201/eid1003.030493.
We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
我们评估了一种基于病毒感染细胞的间接免疫荧光试验,用于检测抗严重急性呼吸综合征相关冠状病毒(SARS-CoV)免疫球蛋白(Ig)G抗体。所有确诊的SARS病例均显示血清转化或IgG抗体滴度呈四倍升高;无对照呈阳性。该试验的敏感性和特异性均为100%。免疫荧光试验可确定SARS-CoV感染状况。