Lord V R, Cherwonogrodzky J W
Instituto de Investigaciones Veterinarias, Centro Nacional de Investigaciones Agropecuarias CENIAP, Laboratorio de Brucellosis, Maracay, Venezuela.
Am J Vet Res. 1992 Mar;53(3):389-91.
In Venezuela, 1,012 cattle sera were screened for their ability to precipitate Brucella melitensis 16M smooth-lipopolysaccharide (S-LPS), B melitensis B115 polysaccharide B (poly B), B abortus 1119-3 O-polysaccharide (PS), or B abortus 1119-3 cyclic 1,2 linked beta-D-glucan (beta-glucan) in an agar-gel immunodiffusion assay. These sera were previously classified as being Brucella abortus-infected, S-19-vaccinated, or negative after an assessment of historical records and results of 5 standard serologic tests. Most of the sera (85%) from infected cattle precipitated S-LPS, poly B, and PS. Serologic results for poly B and PS were identical. On the other hand, 13% of the sera from vaccinated cattle precipitated S-LPS, but none of these sera precipitated poly B or PS. It was concluded that purified PS can alternate with poly B as an antigen to differentiate sera of B abortus-infected from B abortus S-19-vaccinated cattle. None of these sera precipitated beta-glucan.
在委内瑞拉，采用琼脂凝胶免疫扩散试验，对1012份牛血清进行检测，以评估其对布鲁氏菌16M光滑型脂多糖（S-LPS）、布鲁氏菌B115多糖B（多聚B）、流产布鲁氏菌1119-3 O-多糖（PS）或流产布鲁氏菌1119-3环状1,2连接的β-D-葡聚糖（β-葡聚糖）的沉淀能力。在评估历史记录和5项标准血清学检测结果后，这些血清先前被分类为感染流产布鲁氏菌、接种S-19疫苗或呈阴性。大多数来自感染牛的血清（85%）沉淀出S-LPS、多聚B和PS。多聚B和PS的血清学结果相同。另一方面，13%来自接种疫苗牛的血清沉淀出S-LPS，但这些血清均未沉淀出多聚B或PS。得出的结论是，纯化的PS可替代多聚B作为抗原，以区分感染流产布鲁氏菌的牛血清和接种流产布鲁氏菌S-19疫苗的牛血清。这些血清均未沉淀出β-葡聚糖。
