Chen Wen G, White Forest M
Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Expert Rev Proteomics. 2004 Oct;1(3):343-54. doi: 10.1586/14789450.1.3.343.
Protein phosphorylation events are key regulators of cellular signaling processes. In the era of functional genomics, rational drug design programs demand large-scale high-throughput analysis of signal transduction cascades. Significant improvements in the area of mass spectrometry-based proteomics have provided exciting opportunities for rapid progress toward global protein phosphorylation analysis. This review summarizes several recent advances made in the field of phosphoproteomics with an emphasis placed on mass spectrometry instrumentation, enrichment methods and quantification strategies. In the near future, these technologies will provide a tool that can be used for quantitative investigation of signal transduction pathways to generate new insights into biologic systems.
蛋白质磷酸化事件是细胞信号转导过程的关键调节因子。在功能基因组学时代，合理的药物设计方案需要对信号转导级联进行大规模高通量分析。基于质谱的蛋白质组学领域的重大进展为在全球蛋白质磷酸化分析方面迅速取得进展提供了令人兴奋的机会。本综述总结了磷酸化蛋白质组学领域最近取得的一些进展，重点放在质谱仪器、富集方法和定量策略上。在不久的将来，这些技术将提供一种工具，可用于对信号转导途径进行定量研究，从而对生物系统产生新的见解。
