Venier Paola, Zampieron Claudia
Department of Biology, University of Padova, Via Bassi 58/B, 35131 Padova, Italy.
Environ Int. 2005 Sep;31(7):1053-64. doi: 10.1016/j.envint.2005.05.016.
The presence of genetic damage has been investigated in two native species of the Venice lagoon: the common mussel Mytilus galloprovincialis and the grass goby Zosterisessor ophiocephalus. Two sampling campaigns were performed in summer 1998 and 1999. Aromatic-like DNA adducts were analysed in selected tissues of gobies and mussels by using the 32P-postlabelling assay. In 1999, micronuclei and other nuclear abnormalities were additionally scored on gill cells and haemocytes of individual mussels whereas inorganic (As, Cd, Cr, Hg, Ni, Pb, Sn) as well as organic contaminants (polycyclic aromatic hydrocarbons, polychlorinated biphenyls and other chlorinated compounds) were measured in the total mussel pulp. Compared to the lagoon inlet area, gobies and mussels from the industrial district (Marghera) showed significant DNA adduct levels and increased frequencies of cytogenetic alterations (evidence of genetic damage was absent or inconsistent in other sites). The substantial levels of aromatic and chlorinated contaminants detected in mussels from Marghera also support the exposure of native organisms to genotoxic agents.
普通贻贝（Mytilus galloprovincialis）和草虾虎鱼（Zosterisessor ophiocephalus）。在1998年和1999年夏季进行了两次采样活动。通过使用32P后标记分析法，对虾虎鱼和贻贝的选定组织中的类芳香族DNA加合物进行了分析。1999年，还对单个贻贝的鳃细胞和血细胞中的微核及其他核异常情况进行了评分，同时测定了整个贻贝肉中的无机污染物（砷、镉、铬、汞、镍、铅、锡）以及有机污染物（多环芳烃、多氯联苯和其他氯化化合物）。与泻湖入口区域相比，来自工业区（马尔盖拉）的虾虎鱼和贻贝显示出显著的DNA加合物水平以及细胞遗传学改变频率增加（在其他地点不存在或不一致的遗传损伤证据）。在马尔盖拉贻贝中检测到的大量芳香族和氯化污染物也支持本地生物接触到了基因毒性剂。
