Departamento de Biologia Geral, Instituto de Biologia Universidade Federal da Bahia.
Genet Mol Biol. 2009 Apr;32(2):394-8. doi: 10.1590/S1415-47572009000200029. Epub 2009 Jun 1.
The micronucleus and nuclear abnormalities assays have been used increasingly to evaluate genotoxicity of many compounds in polluted aquatic ecossystems. The aim of this study is to verify the efficiency of the micronucleus assay and nuclear abnormality assay in field and laboratory work, when using erythrocytes of the tropical marine fish Bathygobius soporator as genotoxicity biomarkers. Gill peripheral blood samples were obtained from specimens of Bathygobius soporator. In order to investigate the frequencies of micronuclei and to assess the sensitivity of species, the results were compared with samples taken at the reference site and maintained in the laboratory, and fish treated with cyclophosphamide. The micronucleus assay was efficient in demonstrating field pollution and reproducing results in the labotatory. There were significant higher frequencies of micronuclei in two sites subject to discharge of urban and industrial effluents. The nuclear abnormality assay did not appear to be an efficient tool for genotoxicity evaluation when compared with field samples taken at a reference site in laboratory, with a positive control.
微核和核异常检测法已被越来越多地用于评估污染水生生态系统中许多化合物的遗传毒性。本研究旨在验证使用热带海洋鱼类巴氏盔鮋（Bathygobius soporator）的红细胞作为遗传毒性生物标志物时，该检测法在现场和实验室工作中的效率。从巴氏盔鮋的鳃外周血样本中获取样本。为了调查微核的频率并评估物种的敏感性，将结果与取自参比位点并在实验室中保存的样本以及用环磷酰胺处理的鱼类进行比较。微核检测法在证明现场污染和在实验室重现结果方面非常有效。在两个受到城市和工业废水排放影响的地点，微核的频率明显更高。与实验室中参比位点的现场样本和阳性对照相比，核异常检测法似乎不是一种有效的遗传毒性评估工具。
