对质粒pJHCMW1编码的氨基糖苷类6'-N-乙酰基转移酶Ib型中参与乙酰辅酶A结合的保守区域进行诱变分析。
Mutagenesis analysis of a conserved region involved in acetyl coenzyme A binding in the aminoglycoside 6'-N-acetyltransferase type Ib encoded by plasmid pJHCMW1.
作者信息
Pourreza Atousa, Witherspoon Mavee, Fox Jessica, Newmark Jason, Bui Duyen, Tolmasky Marcelo E
机构信息
Department of Biological Science, School of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California 92834-6850, USA.
出版信息
Antimicrob Agents Chemother. 2005 Jul;49(7):2979-82. doi: 10.1128/AAC.49.7.2979-2982.2005.
Abstract
Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6'-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of resistance to kanamycin and amikacin. The replacement of two amino acids, D117 or L120, with alanine residues resulted in complete loss of the resistance phenotype.
摘要
对pJHCMW1编码的I b型氨基糖苷6'-N-乙酰基转移酶中基序A进行丙氨酸扫描，确定了对于该酶赋予对卡那霉素和阿米卡星野生型耐药水平的能力至关重要的氨基酸。用丙氨酸残基取代两个氨基酸D117或L120，导致耐药表型完全丧失。
相似文献
1
Mutagenesis analysis of a conserved region involved in acetyl coenzyme A binding in the aminoglycoside 6'-N-acetyltransferase type Ib encoded by plasmid pJHCMW1.对质粒pJHCMW1编码的氨基糖苷类6'-N-乙酰基转移酶Ib型中参与乙酰辅酶A结合的保守区域进行诱变分析。
Antimicrob Agents Chemother. 2005 Jul;49(7):2979-82. doi: 10.1128/AAC.49.7.2979-2982.2005.
2
Systematic analysis of a conserved region of the aminoglycoside 6'-N-acetyltransferase type Ib.对I b型氨基糖苷6'-N-乙酰基转移酶保守区域的系统分析。
Antimicrob Agents Chemother. 2001 Dec;45(12):3287-92. doi: 10.1128/AAC.45.12.3287-3292.2001.
3
4
External guide sequences targeting the aac(6')-Ib mRNA induce inhibition of amikacin resistance.靶向aac(6')-Ib mRNA的外部引导序列可诱导对阿米卡星耐药性的抑制。
Antimicrob Agents Chemother. 2007 Jun;51(6):1918-25. doi: 10.1128/AAC.01500-06. Epub 2007 Mar 26.
5
Aminoglycoside resistance resulting from tight drug binding to an altered aminoglycoside acetyltransferase.由于药物与改变的氨基糖苷类乙酰转移酶紧密结合而导致的氨基糖苷类耐药性。
Antimicrob Agents Chemother. 2003 May;47(5):1577-83. doi: 10.1128/AAC.47.5.1577-1583.2003.
6
The major aminoglycoside-modifying enzyme AAC(3)-II found in Escherichia coli determines a significant disparity in its resistance to gentamicin and amikacin in China.在中国，大肠埃希菌中主要的氨基糖苷类修饰酶 AAC(3)-II 导致其对庆大霉素和阿米卡星的耐药性存在显著差异。
Microb Drug Resist. 2012 Feb;18(1):42-6. doi: 10.1089/mdr.2010.0190. Epub 2011 Nov 8.
7
Chromosomal origin of acetyltransferase AAC(6') specifying amikacin resistance in Serratia marcescens.粘质沙雷氏菌中赋予阿米卡星抗性的乙酰转移酶AAC(6')的染色体起源
Microbiologia. 1987 Oct;3(3):185-94.
8
Effects of F171 mutations in the 6'-N-acetyltransferase type Ib [AAC(6')-Ib] enzyme on susceptibility to aminoglycosides.6'-N-乙酰基转移酶Ib型【AAC(6')-Ib】中F171突变对氨基糖苷类药物敏感性的影响。
Antimicrob Agents Chemother. 1999 Nov;43(11):2811-2. doi: 10.1128/AAC.43.11.2811.
9
RGFGIGS is an amino acid sequence required for acetyl coenzyme A binding and activity of human spermidine/spermine N1acetyltransferase.RGFGIGS是人类亚精胺/精胺N1 - 乙酰基转移酶结合乙酰辅酶A及发挥活性所必需的氨基酸序列。
J Biol Chem. 1996 Aug 2;271(31):18920-4. doi: 10.1074/jbc.271.31.18920.
10
Molecular cloning of amikacin resistance determinants from a Klebsiella pneumoniae plasmid.肺炎克雷伯菌质粒中阿米卡星耐药决定子的分子克隆
Antimicrob Agents Chemother. 1986 Aug;30(2):315-20. doi: 10.1128/AAC.30.2.315.
引用本文的文献
1
Metabolic regulation of proteome stability via N-terminal acetylation controls male germline stem cell differentiation and reproduction.通过 N 端乙酰化对蛋白质组稳定性的代谢调控控制雄性生殖干细胞的分化和生殖。
Nat Commun. 2023 Oct 23;14(1):6737. doi: 10.1038/s41467-023-42496-9.
2
Novel function of -acetyltransferase for microtubule stability and JNK signaling in organ development.乙酰基转移酶在微管稳定性和 JNK 信号通路中的新功能及其在器官发育中的作用
Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). doi: 10.1073/pnas.2010140118.
3
Structural characterization of a GNAT family acetyltransferase from Elizabethkingia anophelis bound to acetyl-CoA reveals a new dimeric interface.从嗜人按蚊伊丽莎白菌中结合乙酰辅酶 A 的 GNAT 家族乙酰转移酶的结构特征揭示了一个新的二聚体界面。
Sci Rep. 2021 Jan 14;11(1):1274. doi: 10.1038/s41598-020-79649-5.
4
Small Plasmids: Neglected Contributors to Antibiotic Resistance.小型质粒：抗生素耐药性中被忽视的因素
Front Microbiol. 2019 Sep 20;10:2182. doi: 10.3389/fmicb.2019.02182. eCollection 2019.
5
Amikacin: Uses, Resistance, and Prospects for Inhibition.阿米卡星：用途、耐药性和抑制前景。
Molecules. 2017 Dec 19;22(12):2267. doi: 10.3390/molecules22122267.
6
Understanding and overcoming aminoglycoside resistance caused by N-6'-acetyltransferase.了解并克服由N-6'-乙酰基转移酶引起的氨基糖苷类耐药性。
Medchemcomm. 2012 Apr 1;3(4):397-407. doi: 10.1039/C2MD00253A.
7
Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).GCN5相关N-乙酰基转移酶（GNAT）的结构与功能多样性
Int J Mol Sci. 2016 Jun 28;17(7):1018. doi: 10.3390/ijms17071018.
8
Identification of an Inhibitor of the Aminoglycoside 6'--Acetyltransferase type Ib [AAC(6')-Ib] by Glide Molecular Docking.通过Glide分子对接鉴定氨基糖苷6'-N-乙酰转移酶Ib型[AAC(6')-Ib]抑制剂
Medchemcomm. 2016 Jan 1;7(1):184-189. doi: 10.1039/C5MD00316D. Epub 2015 Nov 3.
9
Rise and dissemination of aminoglycoside resistance: the aac(6')-Ib paradigm.氨基糖苷类耐药性的兴起和传播：aac(6')-Ib 范例。
Front Microbiol. 2013 May 17;4:121. doi: 10.3389/fmicb.2013.00121. eCollection 2013.
10
Aminoglycoside modifying enzymes.氨基糖苷修饰酶。
Drug Resist Updat. 2010 Dec;13(6):151-71. doi: 10.1016/j.drup.2010.08.003. Epub 2010 Sep 15.
本文引用的文献
1
Structure and functions of the GNAT superfamily of acetyltransferases.乙酰转移酶GNAT超家族的结构与功能
Arch Biochem Biophys. 2005 Jan 1;433(1):212-26. doi: 10.1016/j.abb.2004.09.003.
2
Molecular mechanism of the enterococcal aminoglycoside 6'-N-acetyltransferase': role of GNAT-conserved residues in the chemistry of antibiotic inactivation.肠球菌氨基糖苷 6'-N-乙酰基转移酶的分子机制：GNAT 保守残基在抗生素失活化学过程中的作用
Biochemistry. 2004 Jan 20;43(2):446-54. doi: 10.1021/bi035667n.
3
Determining the limits of the evolutionary potential of an antibiotic resistance gene.确定抗生素抗性基因进化潜力的限度。
Mol Biol Evol. 2003 Apr;20(4):653-9. doi: 10.1093/molbev/msg074. Epub 2003 Apr 2.
4
X-ray structure of the AAC(6')-Ii antibiotic resistance enzyme at 1.8 A resolution; examination of oligomeric arrangements in GNAT superfamily members.分辨率为1.8埃的AAC(6')-Ii抗生素抗性酶的X射线结构；GNAT超家族成员中寡聚排列的研究。
Protein Sci. 2003 Mar;12(3):426-37. doi: 10.1110/ps.0233503.
5
The molecular basis of the expansive substrate specificity of the antibiotic resistance enzyme aminoglycoside acetyltransferase-6'-aminoglycoside phosphotransferase-2". The role of ASP-99 as an active site base important for acetyl transfer.抗生素抗性酶氨基糖苷乙酰转移酶-6'-氨基糖苷磷酸转移酶-2"的扩展底物特异性的分子基础。天冬酰胺-99作为对乙酰转移重要的活性位点碱基的作用。
J Biol Chem. 2003 Apr 11;278(15):12873-80. doi: 10.1074/jbc.M211680200. Epub 2003 Feb 3.
6
Complete nucleotide sequence of Klebsiella pneumoniae multiresistance plasmid pJHCMW1.肺炎克雷伯菌多重耐药质粒pJHCMW1的全核苷酸序列
Antimicrob Agents Chemother. 2002 Nov;46(11):3422-7. doi: 10.1128/AAC.46.11.3422-3427.2002.
7
Systematic analysis of a conserved region of the aminoglycoside 6'-N-acetyltransferase type Ib.对I b型氨基糖苷6'-N-乙酰基转移酶保守区域的系统分析。
Antimicrob Agents Chemother. 2001 Dec;45(12):3287-92. doi: 10.1128/AAC.45.12.3287-3292.2001.
8
Kinetic and mutagenic characterization of the chromosomally encoded Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase.染色体编码的肠炎沙门氏菌AAC(6')-Iy氨基糖苷N-乙酰转移酶的动力学和诱变特性
Biochemistry. 2001 Mar 27;40(12):3700-9. doi: 10.1021/bi002736e.
9
GCN5-related N-acetyltransferases: a structural overview.GCN5相关的N-乙酰基转移酶：结构概述。
Annu Rev Biophys Biomol Struct. 2000;29:81-103. doi: 10.1146/annurev.biophys.29.1.81.
10
Cascaded multiple classifiers for secondary structure prediction.用于二级结构预测的级联多分类器。
Protein Sci. 2000 Jun;9(6):1162-76. doi: 10.1110/ps.9.6.1162.
Zotero 插件