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对质粒pJHCMW1编码的氨基糖苷类6'-N-乙酰基转移酶Ib型中参与乙酰辅酶A结合的保守区域进行诱变分析。

Mutagenesis analysis of a conserved region involved in acetyl coenzyme A binding in the aminoglycoside 6'-N-acetyltransferase type Ib encoded by plasmid pJHCMW1.

作者信息

Pourreza Atousa, Witherspoon Mavee, Fox Jessica, Newmark Jason, Bui Duyen, Tolmasky Marcelo E

机构信息

Department of Biological Science, School of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California 92834-6850, USA.

出版信息

Antimicrob Agents Chemother. 2005 Jul;49(7):2979-82. doi: 10.1128/AAC.49.7.2979-2982.2005.

DOI:10.1128/AAC.49.7.2979-2982.2005
PMID:15980378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168681/
Abstract

Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6'-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of resistance to kanamycin and amikacin. The replacement of two amino acids, D117 or L120, with alanine residues resulted in complete loss of the resistance phenotype.

摘要

对pJHCMW1编码的I b型氨基糖苷6'-N-乙酰基转移酶中基序A进行丙氨酸扫描，确定了对于该酶赋予对卡那霉素和阿米卡星野生型耐药水平的能力至关重要的氨基酸。用丙氨酸残基取代两个氨基酸D117或L120，导致耐药表型完全丧失。

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本文引用的文献

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Molecular mechanism of the enterococcal aminoglycoside 6'-N-acetyltransferase': role of GNAT-conserved residues in the chemistry of antibiotic inactivation.肠球菌氨基糖苷 6'-N-乙酰基转移酶的分子机制：GNAT 保守残基在抗生素失活化学过程中的作用
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