Khakhria R, Lior H
National Reference Service for Campylobacters, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
Epidemiol Infect. 1992 Jun;108(3):403-14. doi: 10.1017/s0950268800049918.
The extended phage-typing scheme described for Campylobacter jejuni and Campylobacter coli has established 46 different phage types using 19 typing phages. Altogether 754 campylobacter isolates, 672 C. jejuni and 82 C. coli, isolated from human and non-human sources received from 17 different countries were phage-typed. Overall, 80.6% of the total isolates were typable. Among typable strains, 9 phage types (3, 5, 10, 11, 18, 19, 23, 26 and 44) represented 57.0% of the strains, 21.3% of the strains belonged to another 37 phage types and the remaining 2.3% of isolates were designated atypical. The most common phage type 11 (140/754) was frequently observed among C. jejuni isolates from human (113/561) and non-human sources (18/111), whereas type 44 was frequent among C. coli isolates from human (22/59) and from non-human sources (8/23). A study of the animal host-associations of common phage types showed that contaminated cattle and poultry appear to be the most common sources of human infection. The greatest variety of phage types was observed in Canada (24 phage types), followed by Portugal (17 types) and the UK (14 types), reflecting the larger sample sizes from these countries. Phage type 11 was encountered in 12 different countries and prevalence of other phage types varied from one country to another. The number of isolates typable with the scheme varied from 93.2% (261/280) in Canada to 61% (47/77) in Thailand. However, the number and diversity of phage types makes phage typing the method of choice in epidemiological studies of campylobacter infections.
针对空肠弯曲菌和结肠弯曲菌描述的扩展噬菌体分型方案，使用19种分型噬菌体确定了46种不同的噬菌体类型。对从17个不同国家收到的、分离自人类和非人类来源的总共754株弯曲菌进行了噬菌体分型，其中672株为空肠弯曲菌，82株为结肠弯曲菌。总体而言，80.6%的分离株可分型。在可分型菌株中，9种噬菌体类型（3、5、10、11、18、19、23、26和44）占菌株的57.0%，21.3%的菌株属于另外37种噬菌体类型，其余2.3%的分离株被定为非典型。最常见的噬菌体类型11（140/754）在分离自人类（113/561）和非人类来源（18/111）的空肠弯曲菌分离株中经常出现，而44型在分离自人类（22/59）和非人类来源（8/23）的结肠弯曲菌分离株中很常见。对常见噬菌体类型与动物宿主关联的研究表明，受污染的牛和家禽似乎是人类感染最常见的来源。在加拿大观察到的噬菌体类型最多（24种），其次是葡萄牙（17种）和英国（14种），这反映了这些国家的样本量较大。在12个不同国家发现了噬菌体类型11，其他噬菌体类型的流行率因国家而异。该方案可分型的分离株数量从加拿大的93.2%（261/280）到泰国的61%（47/77）不等。然而，噬菌体类型的数量和多样性使得噬菌体分型成为弯曲菌感染流行病学研究中的首选方法。
