Bio-resource Collection and Research Center, Food Industry Research and Development Institute, HsinChu, 300, Taiwan.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1168-1172. doi: 10.1099/ijs.0.014803-0. Epub 2009 Aug 10.
Two actinomycete strains, 0351M-12(T) and 0385M-1(T), were isolated from sediment samples collected from Yilan County and Dali City in Taiwan, respectively. The two isolates displayed characteristics which were similar to those of the genus Herbidospora. They both produced branched and unfragmented substrate mycelia, and aerial hyphae were not produced on Bennett's, glucose-asparagine, Hickey-Tresner, sucrose-nitrate, yeast extract-malt extract, or inorganic salts-starch agars. Short spore-chains were borne on the tips of sporophores arising from the substrate mycelia on oatmeal and oatmeal-nitrate agars. The spore chains contained 10-20 non-motile, smooth-surfaced, oval spores. Galactose, glucose, mannose and madurose were the whole-cell sugars in both strains and meso-diaminopimelic acid was present in their peptidoglycans. The phospholipid pattern was PIV. The predominant menaquinone was MK-10(H(4)) in both strains. Mycolic acids were not detected. Major cellular fatty acids were iso-C(16 : 0) and 10-methyl C(17 : 0). The DNA G+C contents of the two new isolates were 70.6 mol% (0351M-12(T)) and 70.7 mol% (0385M-1(T)), respectively. The relatedness between the isolates and Herbidospora cretacea NBRC 15474(T) was 40.7-48.5 %. The value of DNA-DNA hybridization between strain 0351M-12(T) and strain 0385M-1(T) was 53.6-54.9 %. These two isolates could also be distinguished from each other and from H. cretacea NBRC 15474(T) by differences in several phenotypic characteristics. We therefore propose the names Herbidospora yilanensis sp. nov. and Herbidospora daliensis sp. nov. for these novel bacteria, with strains 0351M-12(T) (=FIRDI 003(T) =BCRC 16875(T) =LMG 24337(T)) and 0385M-1(T) (=FIRDI 004(T) =BCRC 16876(T) =LMG 24336(T)) as the type strains, respectively.
两株放线菌菌株 0351M-12(T)和 0385M-1(T)分别从台湾宜兰县和大理市的沉积物样本中分离得到。这两个分离物表现出与 Herbidospora 属相似的特征。它们都产生分支且不分节的基质菌丝，在 Bennett's、葡萄糖-天冬酰胺、Hickey-Tresner、蔗糖-硝酸盐、酵母提取物-麦芽提取物或无机盐-淀粉琼脂上不产生气生菌丝。短孢子链在来自基质菌丝的分生孢子梗的顶端产生，在燕麦片和燕麦片-硝酸盐琼脂上产生。孢子链包含 10-20 个非运动、光滑表面、椭圆形孢子。两种菌株的整个细胞糖均为半乳糖、葡萄糖、甘露糖和马杜糖，其肽聚糖中含有 meso-二氨基庚二酸。磷脂图谱为 PIV。两种菌株的主要类异戊二烯醌均为 MK-10(H(4))。两种菌株均未检测到类脂酸。主要细胞脂肪酸为 iso-C(16:0)和 10-甲基 C(17:0)。两个新分离株的 DNA G+C 含量分别为 70.6 mol%（0351M-12(T)）和 70.7 mol%（0385M-1(T)）。两个分离株与 Herbidospora cretacea NBRC 15474(T)的相关性为 40.7-48.5%。菌株 0351M-12(T)和菌株 0385M-1(T)之间的 DNA-DNA 杂交值为 53.6-54.9%。这两个分离株也可以通过一些表型特征的差异与彼此和与 Herbidospora cretacea NBRC 15474(T)区分开来。因此，我们提议将这些新型细菌命名为 Herbidospora yilanensis sp. nov. 和 Herbidospora daliensis sp. nov.，其模式菌株分别为 0351M-12(T)（=FIRDI 003(T) =BCRC 16875(T) =LMG 24337(T)）和 0385M-1(T)（=FIRDI 004(T) =BCRC 16876(T) =LMG 24336(T)）。
