Harashima Nanae, Tanosaki Ryuji, Shimizu Yukiko, Kurihara Kiyoshi, Masuda Takao, Okamura Jun, Kannagi Mari
Department of Immunotherapeutics, Tokyo Medical and Dental University, Medical Research Division, Bunkyo-ku, Japan.
J Virol. 2005 Aug;79(15):10088-92. doi: 10.1128/JVI.79.15.10088-10092.2005.
We previously reported that Tax-specific CD8(+) cytotoxic T lymphocytes (CTLs), directed to single epitopes restricted by HLA-A2 or A24, expanded in vitro and in vivo in peripheral blood mononuclear cells (PBMC) from some adult T-cell leukemia (ATL) patients after but not before allogeneic hematopoietic stem cell transplantation (HSCT). Here, we demonstrated similar Tax-specific CTL expansion in PBMC from another post-HSCT ATL patient without HLA-A2 or A24, whose CTLs equally recognized two newly identified epitopes, Tax88-96 and Tax272-280, restricted by HLA-A11, suggesting that these immunodominant Tax epitopes are present in the ATL patient in vivo.
我们之前报道过，针对受HLA - A2或A24限制的单个表位的Tax特异性CD8(+)细胞毒性T淋巴细胞（CTL），在一些成人T细胞白血病（ATL）患者的外周血单个核细胞（PBMC）中，于异基因造血干细胞移植（HSCT）后而非移植前在体外和体内得到扩增。在此，我们在另一位无HLA - A2或A24的HSCT后ATL患者的PBMC中证实了类似的Tax特异性CTL扩增，其CTL同样识别受HLA - A11限制的两个新鉴定的表位Tax88 - 96和Tax272 - 280，这表明这些免疫显性Tax表位在ATL患者体内存在。
