Whitehouse C A, Edwards A A, Tawn E J, Stephan G, Oestreicher U, Moquet J E, Lloyd D C, Roy L, Voisin P, Lindholm C, Barquinero J, Barrios L, Caballin M R, Darroudi F, Fomina J
Westlakes Research Institute, Cumbria, UK.
Int J Radiat Biol. 2005 Feb;81(2):139-45. doi: 10.1080/09553000500103082.
To record the latest information on control levels of translocations in cultured human lymphocytes.
Control-level data from seven European laboratories that are using fluorescence in situ hybridization (FISH) techniques for retrospective biological dosimetry have been combined in a meta-analysis. After correction for the differing probe combinations used, tests of consistency are performed. The combined data have been used to test for individual variation, systematic variation with age, gender and smoking habits.
There is a strong variation of translocation yield with age but no variation was detectable with gender or smoking habits. After correction for age, homogeneity tests showed that about 10% of individuals were outside the 95% confidence limits as opposed to 5% expected. From a total of 385, there is an excess of about 20 individuals most of whom have an unexpectedly high yield of translocations.
For retrospective biological dosimetry purposes a generic age-dependent control level can be assumed. No other lifestyle factors such as smoking appear to have a significant effect on translocation yield.
记录培养的人类淋巴细胞中易位控制水平的最新信息。
来自七个欧洲实验室的控制水平数据已合并进行荟萃分析，这些实验室使用荧光原位杂交（FISH）技术进行回顾性生物剂量测定。在对所使用的不同探针组合进行校正后，进行一致性检验。合并后的数据用于检验个体差异、随年龄、性别和吸烟习惯的系统差异。
易位率随年龄有很大差异，但未发现与性别或吸烟习惯有关的差异。校正年龄后，同质性检验表明，约10%的个体超出95%置信限，而预期为5%。在总共385人中，约有20人超出预期，其中大多数人的易位率意外地高。
为进行回顾性生物剂量测定，可以假定存在一个与年龄相关的通用控制水平。吸烟等其他生活方式因素似乎对易位率没有显著影响。
