Liu Shi-qing, Ma Yong-gang, Peng Hao, Fan Li
Deparment of Orthopaedics of Renmin Hospital, Wuhan University, China.
Chin J Traumatol. 2005 Aug;8(4):216-9.
To detect the concentration of monocyte chemoattractant protein-1 (MCP-1) in the serum of patients with incomplete spinal cord injury and evaluate its relation with the pathologic classification of the spinal cord injury.
MCP-1 concentration in the serum of patients with incomplete spinal cord injury (iSCI), single spine compression and healthy subjects were detected by ELISA, respectively in the present study and the magnetic resonance imaging data of these patients were studied at the same time on a blind base.
Serum level of MCP-1 in iSCI patients was 428 pg/ml +/- 11 pg/ml by ELISA, which was higher than both that of the patients with single spine compression and of controls, with the concentration of 184 pg/ml +/- 21 pg/ml and 124 pg/ml +/- 15 pg/ml, respectively. There was significant difference between any two groups (P < 0.01). iSCI patients with normal MRI showed a lower serum level of MCP-1 as 312 pg/ml+/- 30 pg/ml. Pathological classification of spinal cord edema and hematoma corresponded to 390 pg/ml +/- 16 pg/ml and 508 pg/ml+/- 24 pg/ml in the concentration of MCP-1.
MCP-1 may induce secondary inflammatory response by recruiting inflammatory cells to the injury site and thus affect the prognosis of spinal cord injury.
检测不完全性脊髓损伤患者血清中单核细胞趋化蛋白-1（MCP-1）的浓度，并评估其与脊髓损伤病理分类的关系。
本研究采用酶联免疫吸附测定法（ELISA）分别检测不完全性脊髓损伤（iSCI）患者、单节段脊柱压缩患者及健康受试者血清中MCP-1的浓度，同时对这些患者的磁共振成像数据进行盲法研究。
ELISA检测显示，iSCI患者血清MCP-1水平为428 pg/ml±11 pg/ml，高于单节段脊柱压缩患者（浓度为184 pg/ml±21 pg/ml）和对照组（浓度为124 pg/ml±15 pg/ml）。任意两组间差异均有统计学意义（P<0.01）。MRI正常的iSCI患者血清MCP-1水平较低，为312 pg/ml±30 pg/ml。脊髓水肿和血肿的病理分类对应的MCP-1浓度分别为390 pg/ml±16 pg/ml和508 pg/ml±24 pg/ml。
MCP-1可能通过募集炎症细胞至损伤部位诱导继发性炎症反应，从而影响脊髓损伤的预后。
