Jiménez-Esquilín A E, Roane T M
Department of Biology, University of Colorado, Campus Box 171, Denver, CO 80217-3364, USA.
J Ind Microbiol Biotechnol. 2005 Aug;32(8):378-81. doi: 10.1007/s10295-005-0007-x. Epub 2005 Sep 29.
The antifungal-producing potential of actinomycete populations from the rhizosphere of low-altitude sagebrush, Artemisia tridentata, has been examined. In a continued investigation of new sources of antifungal-producing microorganisms, this study examined the antifungal-producing potential of actinomycetes from the rhizosphere of high-altitude A. tridentata. With high-altitude sagebrush, rhizosphere soil actinomycete numbers were one to four orders of magnitude higher than those found in nonrhizosphere bulk soils and different from those found with the low-altitude plants. A total of 122 actinomycete isolates was screened against nine fungal species and six bacterial species for the production of antimicrobial compounds. Four rhizosphere isolates, Streptomyces amakusaensis, S. coeruleorubidus, S. hawaiiensis and S. scabies, showed broad-spectrum antifungal activity against three or more fungal species in plate assays. In liquid antagonism assays, mycelium production by Aspergillus niger was reduced by up to 50% by two of the actinomycete isolates. These results demonstrate the potential of rhizosphere microbiology in the search for new antimicrobials.
对来自低海拔三齿蒿（Artemisia tridentata）根际的放线菌群体产生抗真菌物质的潜力进行了研究。在对产生抗真菌物质的微生物新来源的持续调查中，本研究检测了高海拔三齿蒿根际放线菌产生抗真菌物质的潜力。对于高海拔三齿蒿，根际土壤放线菌数量比非根际的大块土壤中的数量高1至4个数量级，且与低海拔植物根际土壤中的数量不同。总共对122株放线菌分离株针对9种真菌和6种细菌进行了抗菌化合物产生情况的筛选。在平板试验中，4株根际分离株，即天草链霉菌（Streptomyces amakusaensis）、蓝红链霉菌（S. coeruleorubidus）、夏威夷链霉菌（S. hawaiiensis）和疮痂链霉菌（S. scabies），对三种或更多种真菌表现出广谱抗真菌活性。在液体拮抗试验中，两种放线菌分离株使黑曲霉的菌丝体产量降低了多达50%。这些结果证明了根际微生物学在寻找新型抗菌剂方面的潜力。
