Antonelli Lis R V, Dutra Walderez O, Almeida Roque P, Bacellar Olivia, Carvalho Edgar M, Gollob Kenneth J
Department of Biochemistry-Immunology, Institute of Biological Sciences-Federal University of Minas Gerais (UFMG-ICB), Av. Antonio Carlos, 6627 Belo Horizonte, MG 31640-970, Brazil.
Immunol Lett. 2005 Nov 15;101(2):226-30. doi: 10.1016/j.imlet.2005.06.004.
Leishmaniasis is an important parasitic disease affecting millions worldwide. In attempts to understand the clinical relevance of immunological measurements as determined using flow cytometry, several immunological phenotypes were determined for a group of well defined human leishmaniasis patients and correlated with clinical measurements of the disease (Montenegro skin test (MST) and lesion area). The analysis demonstrated a positive correlation between the MST size and the frequency of ex vivo recent activated CD4(+) T cells. In contrast, higher frequencies of recent activated CD8(+) T cells were correlated with a smaller MST size. Moreover, a positive correlation was observed between the lesion total area and the frequency of activated CD69(+) (ex vivo) and CD40L(+) (cultured with Leishmania soluble antigen (SLA)) T lymphocytes. Finally, larger lesions were also correlated with a higher frequency of SLA specific inflammatory cytokine (IFN-gamma or TNF-alpha) producing lymphocytes. These studies demonstrate that immunological markers are correlated with clinical indicators of human leishmaniasis and serve to better understand the evolution of this important parasitic disease.
利什曼病是一种影响全球数百万人的重要寄生虫病。为了理解使用流式细胞术测定的免疫检测的临床相关性，对一组明确的人类利什曼病患者测定了几种免疫表型，并将其与该疾病的临床检测指标（蒙氏皮肤试验（MST）和病变面积）相关联。分析表明，MST大小与体外近期活化的CD4(+) T细胞频率呈正相关。相反，近期活化的CD8(+) T细胞频率较高与较小的MST大小相关。此外，病变总面积与活化的CD69(+)（体外）和CD40L(+)（与利什曼原虫可溶性抗原（SLA）一起培养）T淋巴细胞频率之间存在正相关。最后，较大的病变也与产生SLA特异性炎性细胞因子（IFN-γ或TNF-α）的淋巴细胞频率较高相关。这些研究表明，免疫标志物与人类利什曼病的临床指标相关，有助于更好地理解这种重要寄生虫病的发展。
