Bielanska Magdalena, Jin Shaoguang, Bernier Martin, Tan Seang Lin, Ao Asangla
McGill Reproductive Centre, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
Fertil Steril. 2005 Aug;84(2):336-42. doi: 10.1016/j.fertnstert.2005.03.031.
To examine diploid-aneuploid mosaicism in human in vitro cultured blastocysts.
A laboratory study on spare blastocysts from an IVF program.
University hospital laboratory.
PATIENTS(S): Forty-three couples undergoing IVF or intracytoplasmic sperm injection.
INTERVENTION(S): Ninety-one blastocysts were spread for fluorescence in situ hybridization using the HCl-Tween 20 method. A total of 6,664 nuclei were analyzed for aneuploidy using fluorescent DNA probes specific to chromosomes 2, 7, and 18.
MAIN OUTCOME MEASURE(S): The proportion of aneuploid cells within each blastocyst.
RESULTS(S): The incidence of diploid-aneuploid mosaicism among 91 blastocysts examined was 17.6%. All of the mosaic blastocysts were abnormal for only one of the three chromosomes tested, with the incidence of involvement of chromosomes 2, 7, and 18 being 3.3%, 8.8%, and 5.5%, respectively. The majority of the mosaic blastocysts had low proportions of aneuploid cells. Ten of the 16 (62.5%) affected blastocysts were of morphology compatible with implantation.
CONCLUSION(S): A considerable proportion of human IVF blastocysts show a form of mosaicism that has been observed in fetal and placental tissues. This mosaicism often arises at the final stage of preimplantation development in vitro and is present in blastocysts of morphology compatible with implantation.
检测人类体外培养囊胚中的二倍体-非整倍体嵌合体。
对体外受精项目中剩余囊胚进行的实验室研究。
大学医院实验室。
43对接受体外受精或卵胞浆内单精子注射的夫妇。
采用盐酸-吐温20法对91个囊胚进行铺展，用于荧光原位杂交。使用针对2号、7号和18号染色体的荧光DNA探针，共分析了6664个核的非整倍体情况。
每个囊胚中非整倍体细胞的比例。
在检测的91个囊胚中，二倍体-非整倍体嵌合体的发生率为17.6%。所有嵌合囊胚仅在检测的三条染色体中的一条上异常，2号、7号和18号染色体受累的发生率分别为3.3%、8.8%和5.5%。大多数嵌合囊胚中非整倍体细胞的比例较低。16个受影响的囊胚中有10个（62.5%）形态与着床相容。
相当比例的人类体外受精囊胚表现出一种在胎儿和胎盘组织中观察到的嵌合形式。这种嵌合体通常在体外着床前发育的最后阶段出现，并且存在于形态与着床相容的囊胚中。
