Ng Enders K O, Cheng Peter K C, Ng Antia Y Y, Hoang T L, Lim Wilina W L
Virology Division, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, China.
Emerg Infect Dis. 2005 Aug;11(8):1303-5. doi: 10.3201/eid1108.041317.
We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples. This assay was evaluated with samples from H5N1-infected patients and demonstrated greater sensitivity and faster turnaround time than nested RT-PCR.
我们开发了一种灵敏且快速的实时逆转录-聚合酶链反应（RT-PCR）检测方法，用于检测临床样本中的甲型H5N1流感病毒。该检测方法用来自H5N1感染患者的样本进行了评估，结果显示其比巢式RT-PCR具有更高的灵敏度和更快的周转时间。
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