Tobin-Janzen T C, Womack J E
Department of Veterinary Pathobiology, Texas A & M University, College Station 77843.
Immunogenetics. 1992;36(3):157-65. doi: 10.1007/BF00661092.
The immunoglobulin genes have not been genetically characterized as thoroughly in cattle as in other mammals, particularly humans and mice. Comparative gene mapping in mammals suggests that the bovine immunoglobulin heavy chain genes, IGHG4 and IGHM might be syntenic with the FOS oncogene. Interestingly, however, when these genes were assigned to bovine syntenic groups utilizing a panel of bovine: hamster hybrid somatic cells, IGH genes were shown to be syntenic with the FES oncogene rather than FOS. In this study IGH and FES were assigned to Bos taurus chromosome 21 while FOS was assigned to chromosome 10. In addition, bovine-specific immunoglobulin-like sequences were observed in the hybrid somatic cells, and one, IGHML1, was mapped to bovine syntenic group U16. The probes used for somatic-cell mapping were also used to screen a small number of cattle of several different breeds for restriction fragment length polymorphisms. IGHG4 and IGHM were shown to be highly polymorphic, while FOS and FES were not.
与其他哺乳动物（尤其是人类和小鼠）相比，牛的免疫球蛋白基因尚未得到充分的遗传学特征描述。哺乳动物的比较基因图谱表明，牛免疫球蛋白重链基因IGHG4和IGHM可能与FOS癌基因同线。然而，有趣的是，当利用一组牛：仓鼠杂种体细胞将这些基因分配到牛的同线群时，IGH基因显示与FES癌基因同线，而不是FOS。在本研究中，IGH和FES被定位到牛染色体21，而FOS被定位到染色体10。此外，在杂种体细胞中观察到牛特异性免疫球蛋白样序列，其中一个IGHML1被定位到牛同线群U16。用于体细胞定位的探针也用于筛选几个不同品种的少数牛的限制性片段长度多态性。结果显示IGHG4和IGHM具有高度多态性，而FOS和FES则没有。
