Sachindra N M, Bhaskar N, Mahendrakar N S
Department of Meat, Fish and Poultry Technology, Central Food Technological Research Institute, Mysore 570 013, India.
Waste Manag. 2006;26(10):1092-8. doi: 10.1016/j.wasman.2005.07.002. Epub 2005 Aug 29.
Shrimp waste, which is produced in large quantities in the Indian seafood processing industries, is one of the important sources of natural carotenoids. Studies were carried out to assess the extractability of shrimp waste carotenoids in different organic solvents and solvent mixtures and to optimize the extraction conditions for maximum yield. A 50:50 mixture of isopropyl alcohol and hexane gave the highest (43.9 microg/g waste) carotenoid extraction yield compared to acetone, methanol, ethanol, isopropyl alcohol, ethyl acetate, ethyl methyl ketone, petroleum ether, and hexane individually and to a mixture of acetone and hexane. Extraction conditions such as percentage of hexane in the solvent mixture of isopropyl alcohol and hexane, ratio of solvent to waste and number of extractions was optimized using a statistically designed experiment. The optimized conditions for maximum yield of carotenoids were 60% hexane in solvent mixture, solvent mixture to waste ratio of 5:1 in each extraction and three extractions. A regression equation for predicting the carotenoid yield as a function of three processing variable (hexane % in solvent mixture, solvent-to-waste ratio and number of extractions) was derived by statistical analysis, and a model with predictive ability of 0.98 was obtained.
虾废料是印度海产品加工业大量产生的废弃物，是天然类胡萝卜素的重要来源之一。开展了相关研究，以评估虾废料类胡萝卜素在不同有机溶剂及溶剂混合物中的可萃取性，并优化提取条件以实现最大产量。与丙酮、甲醇、乙醇、异丙醇、乙酸乙酯、甲乙酮、石油醚和己烷单独使用以及丙酮和己烷的混合物相比，异丙醇和己烷按50:50比例混合时类胡萝卜素提取产量最高（43.9微克/克废料）。采用统计设计实验对异丙醇和己烷溶剂混合物中己烷的百分比、溶剂与废料的比例以及提取次数等提取条件进行了优化。类胡萝卜素最大产量的优化条件为：溶剂混合物中己烷含量60%，每次提取时溶剂混合物与废料的比例为5:1，提取三次。通过统计分析得出了一个预测类胡萝卜素产量与三个加工变量（溶剂混合物中己烷的百分比、溶剂与废料的比例以及提取次数）之间关系的回归方程，得到了一个预测能力为0.98的模型。
