从糙苏叶中分离得到的两种新型对映-贝壳杉烷型二萜类化合物。

Two novel ent-kauranoid diterpenoids from Isodon japonica leaves.

作者信息

Bai Su-Ping, Wei Qing-Yi, Jin Xiao-Ling, Wu Quan-Xiang, Yang Li

机构信息

National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, PR China.

出版信息

Planta Med. 2005 Aug;71(8):764-9. doi: 10.1055/s-2005-871246.

DOI:10.1055/s-2005-871246

PMID:16142643

Abstract

Two novel ent-kaurane diterpenoids, taihangjaponicain A (1), and taihangjaponicain B ( 2), and nine known diterpenoids, epinodosin (3), oridonin (4), epinodosinol (5), lasiokaurin ( 6), 1alpha- O-beta- D-glucopyranosylenmenol (7), lasiodonin (8), rabdosichuanin D ( 9), shikokianin (10) and rabdoternin A (11) were isolated from I. japonica leaves. The structures of the two new compounds were elucidated using 1-D and 2-D NMR spectroscopy. Compounds 1 and 3 - 11 were tested against HL-60, HO-8910 and A-549 human tumor cells. Compounds 4, 6 and 10 showed significant cytotoxicity against HL-60 cells with IC (50) values of 4.6, 2.0 and 3.4 microM, respectively, and against A-549 cells with IC (50) values of 17.5, 11.4 and 18.8 microM, respectively. Compound 6 exhibited moderate cytotoxicity against HO-8910 cells with an IC (50) value of 17.9 microM.

摘要

从日本香茶菜叶片中分离得到两个新的对映-贝壳杉烷二萜类化合物，太行日本香茶菜素A（1）和太行日本香茶菜素B（2），以及9个已知二萜类化合物，表结香苷（3）、冬凌草甲素（4）、表结香苷醇（5）、毛萼香茶菜素（6）、1α-O-β-D-吡喃葡萄糖基延命草醇（7）、毛萼香茶菜丁素（8）、川鄂香茶菜素D（9）、四国香茶菜素（10）和香茶菜萜内酯A（11）。利用一维和二维核磁共振波谱对这两个新化合物的结构进行了鉴定。对化合物1和3 - 11进行了抗HL-60、HO-8910和A-549人肿瘤细胞的测试。化合物4、6和10对HL-60细胞显示出显著的细胞毒性，IC50值分别为4.6、2.0和3.4 μM，对A-549细胞的IC50值分别为17.5、11.4和18.8 μM。化合物6对HO-8910细胞表现出中等细胞毒性，IC50值为17.9 μM。