Hossain Zakir, Kurihara Hideyuki, Hosokawa Masashi, Takahashi Koretaro
Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan.
In Vitro Cell Dev Biol Anim. 2005 May-Jun;41(5-6):154-9. doi: 10.1290/0409058.1.
Glycolipids should have potential effects as antitumor agents. However, very few studies have examined this property of digalactosyl diacylglycerol (DGDG) and sulfoquinovosyl diacylglycerol (SQDG) on colon cancer cells. Cell viability was determined every 24 h with sodium 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium dye reduction assay up to 72 h. Alkaline phosphatase activity was measured for assessing cell differentiation. Apoptosis was tested with enzyme-linked immunosorbent assay analysis. Growth of Caco-2 cells was inhibited apparently at 48 h after addition of SQDG and at 72 h with DGDG. Alkaline phosphatase activity of Caco-2 cells obviously increased in combination with DGDG or SQDG and sodium butyrate (NaBT) at 72 h, indicating that DGDG and SQDG enhanced cell differentiation induced with NaBT. An increased enrichment factor was found when the cell was treated in combination with DGDG or SQDG and NaBT. These results strongly suggest that DGDG and SQDG should be considered as the leading compounds of potentially useful colon cancer chemotherapy agents when NaBT is combined.
糖脂作为抗肿瘤药物应该具有潜在作用。然而，很少有研究考察二半乳糖基二酰基甘油（DGDG）和磺基喹喔啉基二酰基甘油（SQDG）对结肠癌细胞的这一特性。采用2-（4-碘苯基）-3-（4-硝基苯基）-5-（2,4-二磺酸苯基）-2H-四唑𬭩染料还原法每24小时测定一次细胞活力，直至72小时。通过测量碱性磷酸酶活性来评估细胞分化。用酶联免疫吸附测定分析检测细胞凋亡。添加SQDG后48小时以及添加DGDG后72小时，Caco-2细胞的生长明显受到抑制。72小时时，Caco-2细胞的碱性磷酸酶活性在与DGDG或SQDG以及丁酸钠（NaBT）联合作用时明显增加，表明DGDG和SQDG增强了由NaBT诱导的细胞分化。当细胞与DGDG或SQDG以及NaBT联合处理时，发现富集因子增加。这些结果有力地表明，当与NaBT联合使用时，DGDG和SQDG应被视为潜在有用的结肠癌化疗药物的主要化合物。
