显色培养基在从临床标本中分离酵母菌中的应用。

Use of chromogenic medium in the isolation of yeasts from clinical specimens.

作者信息

Murray C K, Beckius M L, Green J A, Hospenthal D R

机构信息

Department of Medicine, Infectious Disease Service1 and Department of Pathology and Area Laboratory Support2, Brooke Army Medical Center, Fort Sam Houston, TX, USA.

出版信息

J Med Microbiol. 2005 Oct;54(Pt 10):981-985. doi: 10.1099/jmm.0.45942-0.

DOI:10.1099/jmm.0.45942-0

PMID:16157554

Abstract

Over a 1 year period 3296 specimens submitted for fungal culture were plated onto routine mycological media (RM) and CHROMagar Candida (CaC) to evaluate the capability of CaC to improve on RM. With RM, cultures producing single yeast isolates were identified from 802 specimens. CaC produced similar results, with 76 % agreement. Of 761 specimens that yielded a single Candida species by RM, 615 (81 %) produced one or more yeast isolates using CaC. Of concern, 132 negative CaC cultures corresponded to specimens that yielded C. albicans alone on RM. When yeasts were recovered, CaC correctly identified 98 % of C. albicans, 93 % of Candida tropicalis, 96 % of Candida glabrata and 100 % of Candida krusei based on typical colours. CaC did potentially improve on RM by detecting yeasts in 91 specimens that yielded none by routine methods. CaC was noted to recover more yeast isolates than RM when mixed cultures were detected. Overall, the role of CaC in improving RM appears limited.

摘要

在为期1年的时间里，将3296份提交进行真菌培养的标本接种到常规真菌培养基（RM）和科玛嘉念珠菌显色培养基（CaC）上，以评估CaC在改进RM方面的能力。使用RM时，从802份标本中鉴定出产生单一酵母分离株的培养物。CaC产生了类似的结果，一致性为76%。在通过RM产生单一念珠菌属的761份标本中，615份（81%）使用CaC产生了一种或多种酵母分离株。令人担忧的是，132份CaC培养阴性的标本对应于在RM上仅产生白色念珠菌的标本。当分离出酵母时，根据典型颜色，CaC正确鉴定出98%的白色念珠菌、93%的热带念珠菌、96%的光滑念珠菌和100%的克柔念珠菌。CaC确实有可能通过检测出91份常规方法未检测出酵母的标本中的酵母而改进RM。当检测到混合培养物时，发现CaC比RM能分离出更多的酵母分离株。总体而言，CaC在改进RM方面的作用似乎有限。