Daef Enas, Moharram Ahmed, Eldin Salwa Seif, Elsherbiny Nahla, Mohammed Mona
Medical Microbiology and Immunology Department Faculty of Medicine Assiut University Assiut Egypt.
Botany Department Faculty of Science Assiut University Assiut Egypt.
Braz J Microbiol. 2014 May 2;45(1):255-62. doi: 10.1590/S1517-83822014005000040. eCollection 2014.
Identification of Candida cultured from various clinical specimens to the species level is increasingly necessary for clinical laboratories. Although sn PCR identifies the species within hours but its cost-effectiveness is to be considered. So there is always a need for media which help in the isolation and identification at the species level. The study aimed to evaluate the performance of different chromogenic media and to compare the effectiveness of the traditional phenotypic methods vs. seminested polymerase chain reaction (sn PCR) for identification of Candida species. One hundred and twenty seven Candida strains isolated from various clinical specimens were identified by conventional methods, four different chromogenic media and sn PCR. HiCrome Candida Differential and CHROMagar Candida media showed comparably high sensitivities and specificities in the identification of C. albicans, C. tropicalis, C. glabrata and C. krusei. CHROMagar Candida had an extra advantage of identifying all C. parapsilosis isolates. CHROMagar-Pal's medium identified C. albicans, C. tropicalis and C. krusei with high sensitivities and specificities, but couldn't identify C. glabrata or C. parapsilosis. It was the only medium that identified C. dubliniensis with a sensitivity and specificity of 100%. Biggy agar showed the least sensitivities and specificities. The overall concordance of the snPCR compared to the conventional tests including CHROMAgar Candida in the identification of Candida species was 97.5%. The use of CHROMAgar Candida medium is an easy and accurate method for presumptive identification of the most commonly encountered Candida spp.
对于临床实验室而言，将从各种临床标本中培养出的念珠菌鉴定到种水平变得越来越必要。尽管单巢式聚合酶链反应（sn PCR）能在数小时内鉴定出菌种，但其成本效益仍需考虑。因此，始终需要有助于在种水平进行分离和鉴定的培养基。本研究旨在评估不同显色培养基的性能，并比较传统表型方法与半巢式聚合酶链反应（sn PCR）在鉴定念珠菌菌种方面的有效性。通过传统方法、四种不同的显色培养基和sn PCR对从各种临床标本中分离出的127株念珠菌菌株进行了鉴定。HiCrome念珠菌鉴别培养基和CHROMagar念珠菌培养基在白色念珠菌、热带念珠菌、光滑念珠菌和克柔念珠菌的鉴定中显示出相当高的敏感性和特异性。CHROMagar念珠菌培养基具有鉴定所有近平滑念珠菌分离株的额外优势。CHROMagar - Pal培养基在鉴定白色念珠菌、热带念珠菌和克柔念珠菌时具有较高的敏感性和特异性，但无法鉴定光滑念珠菌或近平滑念珠菌。它是唯一能以100%的敏感性和特异性鉴定都柏林念珠菌的培养基。Biggy琼脂显示出最低的敏感性和特异性。与包括CHROMAgar念珠菌在内的传统检测方法相比，snPCR在念珠菌菌种鉴定中的总体一致性为97.5%。使用CHROMAgar念珠菌培养基是一种简单且准确的方法，可用于初步鉴定最常见的念珠菌属菌种。
