Khadka Sundar, Sherchand Jeevan Bahadur, Pokhrel Bharat Mani, Parajuli Keshab, Mishra Shyam Kumar, Sharma Sangita, Shah Niranjan, Kattel Hari Prasad, Dhital Subhash, Khatiwada Sulochana, Parajuli Narayan, Pradhan Manoj, Rijal Basista Prasad
HIV Reference Unit, National Public Health Laboratory, Kathmandu, Nepal.
Department of Microbiology, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
BMC Res Notes. 2017 Jun 24;10(1):218. doi: 10.1186/s13104-017-2547-3.
Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases along with increased resistance to antifungal drugs has made a serious concern. Resistance to antifungal agents has increased during the last decade. Thus, identification of Candida up to species level and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to speciate Candida species and to determine antifungal susceptibility pattern of Candida species to antifungal agents.
A total of 100 consecutive Candida species were isolated from 1248 clinical specimens over 7 months period. Growths on Sabouraud dextrose agar were evaluated for colony appearance, macroscopic examination, Gram staining, germ tube test and urea hydrolysis test. Further, they were processed for Candida speciation on CHROMagar. Antifungal susceptibility testing was performed as recommended by Clinical and Laboratory Standards Institute (CLSI) M44-A document.
Out of 100 Candida isolates, Candida albicans (56%) was the most common species. Among the non-albicans Candida species, Candida tropicalis (20%) was the predominant isolate followed by Candida glabrata (14%). Regarding antifungal susceptibility pattern, Candida species were more susceptible to clotrimazole (82%) followed by fluconazole (64%) and miconazole (44%).
Candida albicans was the predominant species responsible for various Candidal infections. Among commonly used antifungal drugs clotrimazole, miconazole and fluconazole were most effective.
念珠菌属可导致从黏膜皮肤感染到危及生命的侵袭性疾病等各种临床感染，同时其对抗真菌药物的耐药性增加已成为一个严重问题。在过去十年中，对抗真菌药物的耐药性有所增加。因此，将念珠菌鉴定到种水平并进行其抗真菌药敏试验在念珠菌感染的管理中具有至关重要的意义。本研究的目的是对念珠菌进行种属鉴定，并确定念珠菌属对抗真菌药物的药敏模式。
在7个月的时间里，从1248份临床标本中连续分离出100株念珠菌。对沙氏葡萄糖琼脂上的生长物进行菌落外观、宏观检查、革兰氏染色、芽管试验和尿素水解试验评估。此外，在科玛嘉显色培养基上对它们进行念珠菌菌种鉴定处理。按照临床和实验室标准协会（CLSI）M44 - A文件的建议进行抗真菌药敏试验。
在100株念珠菌分离株中，白色念珠菌（56%）是最常见的菌种。在非白色念珠菌中，热带念珠菌（20%）是主要分离株，其次是光滑念珠菌（14%）。关于抗真菌药敏模式，念珠菌对克霉唑（82%）最敏感，其次是氟康唑（64%）和咪康唑（44%）。
白色念珠菌是导致各种念珠菌感染的主要菌种。在常用的抗真菌药物中，克霉唑、咪康唑和氟康唑最为有效。
