Xu Xiuling, Yang Lixin, Xu Xiaoyi, Wang Xin, Chen Xuesi, Liang Qizhi, Zeng Jing, Jing Xiabin
State key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
J Control Release. 2005 Nov 2;108(1):33-42. doi: 10.1016/j.jconrel.2005.07.021. Epub 2005 Sep 13.
Ultrafine fibers containing water-soluble drugs were successfully electrospun from water-in-oil (W/O) emulsions, in which the aqueous phase contained the water-soluble drugs and the oily phase was a chloroform solution of amphiphilic poly (ethylene glycol)-poly (L-lactic acid) (PEG-PLLA) diblock copolymer. The diameter of the electrospun fibers was in the range of 300 nm-1 microm. A water-soluble anticancer agent, doxorubicin hydrochloride (Dox), was used as the model drug. Its content in the fibers was 1-5 wt.% and it was entirely encapsulated inside the electrospun fibers. Its release from the fibers was controlled by the combined diffusion mechanism and enzymatic degradation mechanism. At the early stage, the diffusion mechanism was predominant and a certain time later, the enzymatic degradation mechanism became predominant. The antitumor activity of the Dox incorporated in the PEG-PLLA fibers against mice glioma cells (C6 cell lines) was evaluated by MTT method. The results showed that the Dox could be released from the fibers without losing cytotoxicity.
含水溶性药物的超细纤维由油包水（W/O）乳液成功电纺而成，其中水相含有水溶性药物，油相是两亲性聚（乙二醇）-聚（L-乳酸）（PEG-PLLA）二嵌段共聚物的氯仿溶液。电纺纤维的直径在300纳米至1微米范围内。一种水溶性抗癌剂盐酸阿霉素（Dox）用作模型药物。其在纤维中的含量为1-5 wt.%，且完全包裹在电纺纤维内部。其从纤维中的释放受扩散机制和酶降解机制共同控制。在早期，扩散机制占主导，一段时间后，酶降解机制占主导。通过MTT法评估了掺入PEG-PLLA纤维中的阿霉素对小鼠胶质瘤细胞（C6细胞系）的抗肿瘤活性。结果表明，阿霉素可从纤维中释放且不失细胞毒性。
