Kitahara Maki, Sakamoto Mitsuo, Ike Masako, Sakata Shinji, Benno Yoshimi
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):2143-2147. doi: 10.1099/ijs.0.63788-0.
Nine strains of Gram-negative, anaerobic rod were isolated from human faeces. Based on phylogenetic analysis and specific phenotypic characteristics, these strains were included within the Bacteroides cluster and were divided into two clusters. Strains from the two clusters showed 16S rRNA gene sequence similarities of 90.4 and 92.7% to the nearest recognized species, Bacteroides vulgatus. The strains also formed two clusters exhibiting a 16S rRNA gene sequence divergence of approximately 6%. DNA-DNA hybridization studies confirmed that the two novel strain clusters were distinct from each other. Based on the phenotypic and phylogenetic findings, two novel species, Bacteroides plebeius sp. nov. and Bacteroides coprocola sp. nov., are proposed, each representing one of the two strain clusters. The DNA G+C content of the type strains were 43.9 mol% for B. plebeius (M12(T)=JCM 12973(T)=DSM 17135(T)) and 42.4 mol% for B. coprocola (M16(T)=JCM 12979(T)=DSM 17136(T)).
从人类粪便中分离出9株革兰氏阴性厌氧杆菌。基于系统发育分析和特定的表型特征,这些菌株被归入拟杆菌属群,并分为两个簇。来自这两个簇的菌株与最接近的已知种——普通拟杆菌的16S rRNA基因序列相似性分别为90.4%和92.7%。这些菌株还形成了两个簇,其16S rRNA基因序列差异约为6%。DNA-DNA杂交研究证实这两个新菌株簇彼此不同。基于表型和系统发育研究结果,提出了两个新种,即平民拟杆菌(Bacteroides plebeius sp. nov.)和粪栖拟杆菌(Bacteroides coprocola sp. nov.),每个新种代表两个菌株簇中的一个。平民拟杆菌模式菌株(M12(T)=JCM 12973(T)=DSM 17135(T))的DNA G+C含量为43.9 mol%,粪栖拟杆菌模式菌株(M16(T)=JCM 12979(T)=DSM 17136(T))的DNA G+C含量为42.4 mol%。