Department of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China.
Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China.
J Cell Mol Med. 2022 Dec;26(24):6066-6078. doi: 10.1111/jcmm.17626. Epub 2022 Dec 2.
Chronic kidney disease (CKD) affects approximately 10% of the global population. Muscle atrophy occurs in patients with almost all types of CKD, and the gut microbiome is closely related to protein consumption during chronic renal failure (CRF). This study investigated the effects of Bacteroides plebeius on protein energy consumption in rats with CKD, and our results suggest that Bacteroides plebeius may combat muscle atrophy through the Mystn/ActRIIB/SMAD2 pathway. A total of 5/6 Nx rats were used as a model of muscle wasting in CKD. The rats with muscle wasting were administered Bacteroides plebeius (2 × 10 cfu/0.2 ml) for 8 weeks. The results showed that Bacteroides plebeius administration significantly inhibited muscle wasting in CKD. High-throughput 16 S rRNA pyrosequencing revealed that supplementation with Bacteroides plebeius rescued disturbances in the gut microbiota. Bacteroides plebeius could also enhance the barrier function of the intestinal mucosa. Bacteroides plebeius may modulate the gut microbiome and reduce protein consumption by increasing the abundance of probiotics and reducing damage to the intestinal mucosal barrier. Our findings suggest that Bacteroides plebeius may combat muscle atrophy through the Mystn/ActRIIB/SMAD2 pathway.
慢性肾脏病（CKD）影响全球约 10%的人口。几乎所有类型的 CKD 患者都会出现肌肉萎缩，而肠道微生物组与慢性肾衰竭（CRF）期间的蛋白质消耗密切相关。本研究探讨了普雷沃氏菌属对 CKD 大鼠蛋白质能量消耗的影响，我们的研究结果表明，普雷沃氏菌属可能通过 Mystn/ActRIIB/SMAD2 通路来对抗肌肉萎缩。使用 5/6 Nx 大鼠作为 CKD 肌肉消耗的模型。对肌肉萎缩的大鼠给予普雷沃氏菌属（2×10 个 cfu/0.2 ml）治疗 8 周。结果表明，普雷沃氏菌属的给药显著抑制了 CKD 中的肌肉消耗。高通量 16S rRNA 焦磷酸测序显示，补充普雷沃氏菌属可纠正肠道微生物群的紊乱。普雷沃氏菌属还可以增强肠道黏膜的屏障功能。普雷沃氏菌属可能通过调节肠道微生物组并增加益生菌的丰度和减少对肠道黏膜屏障的损害来减少蛋白质的消耗。我们的研究结果表明，普雷沃氏菌属可能通过 Mystn/ActRIIB/SMAD2 通路来对抗肌肉萎缩。
