从人粪便中分离出的新种费氏拟杆菌。
Bacteroides finegoldii sp. nov., isolated from human faeces.
作者信息
Bakir Mohammad Abdul, Kitahara Maki, Sakamoto Mitsuo, Matsumoto Mitsuharu, Benno Yoshimi
机构信息
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Laboratory of Dairy Science and Technology, Kyodo Milk Industry Co. Ltd, Hinode, Tokyo 190-0182, Japan.
出版信息
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):931-935. doi: 10.1099/ijs.0.64084-0.
Two strains, 199T and 176, of Gram-negative, anaerobic, rod-shaped, non-spore-forming bacteria were isolated during studies of microbiota in human faeces using polyamine-deficient medium. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolated strains belonged to the genus Bacteroides. The G+C content (42.4-43.0 mol%) and major fatty acid composition (anteiso-C15:0, 31.8-36.2 %) supported the affiliation of the two strains to the genus Bacteroides. 16S rRNA gene sequence similarities with their closest neighbours, Bacteroides thetaiotaomicron and Bacteroides ovatus, ranged from 94.6 to 96.5 %. DNA-DNA hybridization and phenotypic analysis showed that the two strains belonged to the same species (DNA-DNA relatedness of 89 %) and were distinct from their close relatives, B. thetaiotaomicron and B. ovatus (<23 % DNA-DNA relatedness). On the basis of these data, a novel species of the genus Bacteroides, Bacteroides finegoldii sp. nov., is proposed, comprising the designated type strain 199T (=JCM 13345T=DSM 17565T) and strain 176 (=JCM 13346).
在使用多胺缺陷培养基对人类粪便微生物群的研究过程中，分离出了两株革兰氏阴性、厌氧、杆状、不形成芽孢的细菌，分别为199T和176。基于16S rRNA基因序列的系统发育分析表明，分离出的菌株属于拟杆菌属。G+C含量（42.4 - 43.0 mol%）和主要脂肪酸组成（anteiso-C15:0，31.8 - 36.2%）支持这两株菌归属于拟杆菌属。与它们最近的亲缘种——多形拟杆菌和卵形拟杆菌的16S rRNA基因序列相似性在94.6%至96.5%之间。DNA-DNA杂交和表型分析表明，这两株菌属于同一物种（DNA-DNA相关性为89%），且与它们的近缘种多形拟杆菌和卵形拟杆菌不同（DNA-DNA相关性<23%）。基于这些数据，提出了拟杆菌属的一个新物种——费氏拟杆菌（Bacteroides finegoldii sp. nov.），包括指定的模式菌株199T（=JCM 13345T=DSM 17565T）和菌株176（=JCM 13346）。
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