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巴西免疫血液学外部质量评估计划的十二年:该计划的益处

Twelve years of the Brazilian External Quality Assessment Program in Immunohematology: benefits of the program.

作者信息

Melo Laércio, Pellegrino Jordão, Bianco Celso, Castilho Lilian

机构信息

Hemocentro da UNICAMP, São Paulo, Brazil.

出版信息

J Clin Lab Anal. 2005;19(5):209-18. doi: 10.1002/jcla.20080.

Abstract

The Brazilian External Quality Assessment Program in Immunohematology (BEQAPI) was introduced with the objective of evaluating the quality of diagnosis in immunohematology. From 1992 to 2003, proficiency tests for ABO grouping, Rh (D, C, c, E, e), K phenotyping, direct antiglobulin testing (DAT), antibody screening (AS), and antibody identification (AI) were performed. A total of 41 evaluations were carried out in 223 institutions. Over the period of 12 years, the program included 8,014 ABO typing, 8,000 RhD typing, 5,193 Rh typing (C, c, E, e), 5,101 K phenotyping, 7,939 AS, 4,533 AI, and 7,912 DATs. Erroneous responses were classified as clerical, technical, or undetermined. A substantial proportion of erroneous responses due to clerical errors occurred in ABO typing (76/76 errors), RhD typing (34/58 errors), and Rh phenotyping (50/73 errors). Technical errors occurred predominantly for weak D (91/95 errors), AS (252/301 errors), and AI (321/335 errors). Based on these results, since 1996, participants have received "Questions and Case Studies" in Immunohematology as an incentive for training and education. The results of the present study show an improvement in the performance of participants in the course of the program. We found that a well-organized external proficiency program can contribute to the improvement of quality of testing in Immunohematology.

摘要

巴西免疫血液学外部质量评估项目(BEQAPI)旨在评估免疫血液学诊断质量。1992年至2003年期间,开展了ABO血型鉴定、Rh(D、C、c、E、e)分型、K抗原表型鉴定、直接抗球蛋白试验(DAT)、抗体筛查(AS)和抗体鉴定(AI)的能力验证测试。共对223家机构进行了41次评估。在这12年期间,该项目包括8014次ABO血型分型、8000次RhD分型、5193次Rh(C、c、E、e)分型、5101次K抗原表型鉴定、7939次AS、4533次AI和7912次DAT。错误回答被分为文书错误、技术错误或无法确定的错误。由于文书错误导致的大量错误回答发生在ABO血型分型(76/76次错误)、RhD分型(34/58次错误)和Rh表型鉴定(50/73次错误)中。技术错误主要发生在弱D(91/95次错误)、AS(252/301次错误)和AI(321/335次错误)中。基于这些结果,自1996年以来,参与者收到了免疫血液学方面的“问题与案例研究”,作为培训和教育的激励措施。本研究结果表明,在该项目过程中参与者的表现有所改善。我们发现,一个组织良好的外部能力验证项目有助于提高免疫血液学检测质量。

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